CAR, Fc Chimera Active Protein

CAR, Fc Chimera, Recombinant, Mouse (Coxsackie Adenovirus Receptor)

Cat. Num. AAA636652

• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/1ug.
• Synonyms: CAR; Fc Chimera; Recombinant; Mouse (Coxsackie Adenovirus Receptor); Fc Chimera active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/1ug.
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS, 0.1% carrier protein to > 0.1mg/ml

• Activity: Measured by the ability of the immobilized protein to support the adhesion of human neutrophils. When 2 x 10e5 cells/well are added to CAR-coated plates (10ug/ml, 100 L/well), 40-60% of the cells will adhere after 20 minutes at 37 degree C.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.; Storage and Stability:; Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile PBS, 0.1% carrier protein. Aliquot and store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
• Preparation and Storage: -20 degree C

Related Product Information for CAR, Fc Chimera active protein

CAR (coxsackie and adenovirus receptor), also known as CXADR, is a 46 kD type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1-3). CAR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CAR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4-6). The 365 amino acid (aa) mouse CAR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). A PDZ binding motif at the C-terminus interacts with several cytoplasmic junctional proteins (1). The ECD of mouse CAR shares 97%, 90%, 89%, 89% and 88% aa sequence identity with the corresponding regions of rat, human, bovine, porcine and canine CAR, respectively. An alternately spliced isoform (CAR2) that diverges in the C-terminal 15 aa shows the same expression pattern, but may show different subcellular localization (4, 8). Transcription of other splice variants has been detected, but not their translation. A secreted form identified in serum and pleural fluid can block viral infection (9).

Product Categories/Family for CAR, Fc Chimera active protein

Molecular Biology; MB-Receptors