Bone Morphogenetic Protein 10 Active Protein | BMP10 active protein

Bone Morphogenetic Protein 10, Recombinant, Human (BMP10)

Cat. Num. AAA650205

• Gene Names: BMP10; MGC126783
• Purity: Highly Purified; 97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1.0 EU/ug o(LAL).
• Synonyms: Bone Morphogenetic Protein 10; Recombinant; Human (BMP10); BMP10 active protein

• Purity/Purification: Highly Purified; 97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1.0 EU/ug o(LAL).
• Form/Format: Supplied as a lyophilized powder from 30% Acetonitrile, 0.1% TFA, 1.25mg BSA. Reconstitute to 10ug/ml in sterile 4mM HCI, with 0.1% HSA or BSA.

• Activity: Measured by its ability to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Mazerbourg, S. et al., 2005, J. Biol. Chem. 280:32122). The ED50 for this effect is typically 15-60ng/mL.
• Reconstitution: It is recommended that sterile 4mM HCI containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/mL.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 4mM HCI, with 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for BMP10 active protein

The mature recombinant human BMP-10, generated by the proteolytic removal of the signal peptide and the propeptide, is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the mature recombinant human BMP-10 starts at Asn 317. The monomer has a calculated molecular mass of 12.2kD and migrates as an approximately 12.2kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for BMP10 active protein

Growth Factors; Cytokines; Growth Factors-BMP

NCBI and Uniprot Product Information

• NCBI GI #: 3873291
• NCBI GeneID: 27302
• UniProt Accession #: O95393; Q53R17; Q6NTE0
• Molecular Weight: 48,047 Da
• NCBI Official Full Name: bone morphogenetic protein 10
• NCBI Official Synonym Full Names: bone morphogenetic protein 10
• NCBI Official Symbol: BMP10
• NCBI Official Synonym Symbols: MGC126783
• NCBI Protein Information: bone morphogenetic protein 10; BMP-10; OTTHUMP00000159973
• UniProt Protein Name: Bone morphogenetic protein 10
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP10
• UniProt Entry Name: BMP10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq]

Uniprot Description

Function: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Ref.4 Ref.5

Subunit structure: Homodimer; disulfide-linked

Subcellular location: Secreted

Sequence similarities: Belongs to the TGF-beta family.