apo-SERUM AMYLOID A1 Active Protein | SAA1 active protein

RECOMBINANT HUMAN apo-SERUM AMYLOID A1

Cat. Num. AAA232509

• Gene Names: SAA1; SAA; PIG4; SAA2; TP53I4
• Applications: Functional Assay
• Purity: >98% by SDS PAGE and HPLC analysis
• Synonyms: apo-SERUM AMYLOID A1; RECOMBINANT HUMAN apo-SERUM AMYLOID A1; SAA1 active protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 0.1mg/ml after reconstitution (varies by lot)

• Applicable Applications for SAA1 active protein: Functional Assays (FN)
• Reconstitution: Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% BSA is recommended.; Preparation
• Buffer Solution: 5mM Tris, pH7.6
• Activity: Determined by its ability to chemoattract human monocytes using a range of 10.0-10.0 ng/ml.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for SAA1 active protein

Serum Amyloid A (apo-SAA) is an acute phase response protein and injury specific apolipoprotein, synthesized primarily in the liver, which is found in trace amounts in association with high density lipoproteins (HDL) in human serum, but increases dramatically during an acute response to inflammation, infection, trauma, and malignant neoplasia, to become the most abundant HDL apolipoprotein. The acute phase isoforms of apo-SAA are encoded by the genes SAA1 (Apo-SAA1) and SAA2 (Apo-SAA2), and both include allelic variants.

NCBI and Uniprot Product Information

• NCBI GI #: 134167
• NCBI GeneID: 6288
• UniProt Accession #: P02735; P0DJI8
• Molecular Weight: 11.7kD (105 amino acid residues)
• NCBI Official Synonym Full Names: serum amyloid A1
• NCBI Official Symbol: SAA1
• NCBI Official Synonym Symbols: SAA; PIG4; SAA2; TP53I4
• NCBI Protein Information: serum amyloid A-1 protein
• Protein Family: Amyloid protein

NCBI Description

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]

Uniprot Description

SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding

Biological Process: positive regulation of interleukin-1 secretion; neutrophil chemotaxis; platelet activation; receptor-mediated endocytosis; elevation of cytosolic calcium ion concentration; regulation of protein secretion; positive regulation of cell adhesion; negative regulation of inflammatory response; acute-phase response; innate immune response; lymphocyte chemotaxis; macrophage chemotaxis; positive regulation of cytokine secretion