Angiotensin I Converting Enzyme-2, Rat Active Protein | ACE2 active protein

Angiotensin I Converting Enzyme-2, Recombinant, Rat (Ace-2)

Cat. Num. AAA637973

• Gene Names: ACE2; ACEH
• Purity: Purified; 95% as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Angiotensin I Converting Enzyme-2; Rat; Recombinant; Rat (Ace-2); ACE2 active protein

• Purity/Purification: Purified; 95% as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a liquid in 12.5mM Tris, 0.1M NaCl, 0.5uM ZnCl2, pH 8.0, 50% glycerol.

• Specific Activity: 300 pmoles/min/ug.
• Biological Activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH.
• Endotoxin Level: 1EU/ug of the enzyme as determined by the LAL method.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ACE2 active protein

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE.1 The predicted rat ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity.2 Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of cardiovascular function in vivo.3 In addition, ACE-2 has a critical function in acute lung injury.

Product Categories/Family for ACE2 active protein

Molecular Biology; MB-Enzymes; Angiotensin Converting (ACE)

NCBI and Uniprot Product Information

• NCBI GI #: 11225609
• NCBI GeneID: 59272
• NCBI Accession #: NP_068576.1
• NCBI GenBank Nucleotide #: NM_021804.2
• UniProt Accession #: Q9BYF1; Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6; C7ECU1
• Molecular Weight: The 733 amino acid residue rrACE-2 has a predicted molecular mass of approximately 85kD. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is approximately 120kD under reducing and non-reducing conditions.
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: ACE2
• NCBI Official Synonym Symbols: ACEH
• NCBI Protein Information: angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: ACE2
• UniProt Synonym Gene Names: ACEH
• UniProt Entry Name: ACE2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.23; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; integral to membrane; plasma membrane; lipid raft

Molecular Function: viral receptor activity; peptidyl-dipeptidase activity; protein binding; carboxypeptidase activity; metallopeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cytokine production; regulation of cell proliferation; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior