Typical Testing Data/Standard Curve (for reference only)
Monkey Angiotensin I Converting Enzyme 2 ELISA Kit | ACE2 elisa kit
Monkey Angiotensin I Converting Enzyme 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ACE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACE2 antibody and an ACE2-HRP conjugate. The assay sample and buffer are incubated together with ACE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACE2 concentration since ACE2 from samples and ACE2-HRP conjugate compete for the anti-ACE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACE2 from the sample, fewer sites are left to bind ACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Uniprot Description
ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Protease; EC 3.4.17.23
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; integral to membrane; plasma membrane; lipid raft
Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; carboxypeptidase activity; metallopeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding
Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cytokine production; regulation of cell proliferation; cellular protein metabolic process; regulation of vasoconstriction; angiotensin catabolic process in blood; regulation of inflammatory response; receptor biosynthetic process; angiotensin mediated drinking behavior
Research Articles on ACE2
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Product Notes
The Monkey ACE2 ace2 (Catalog #AAA741509) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741509 ELISA Kit recognizes Monkey ACE2. It is sometimes possible for the material contained within the vial of "Angiotensin I Converting Enzyme 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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