Goat Angiotensin I Converting Enzyme ELISA Kit | ACE elisa kit

Goat Angiotensin I Converting Enzyme ELISA Kit

Cat. Num. AAA742294

• Gene Names: ACE; DCP; ICH; ACE1; DCP1; CD143; MVCD3
• Reactivity: Goat
• Synonyms: Angiotensin I Converting Enzyme; Goat Angiotensin I Converting Enzyme ELISA Kit; ACE elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This ACE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat ACE. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACE elisa kit

Principle of the Assay: ACE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACE antibody and an ACE-HRP conjugate. The assay sample and buffer are incubated together with ACE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACE concentration since ACE from samples and ACE-HRP conjugate compete for the anti-ACE antibody binding site. Since the number of sites is limited, as more sites are occupied by ACE from the sample, fewer sites are left to bind ACE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ACE elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 76057114
• NCBI GeneID: 1636
• Molecular Weight: 78,694 Da
• NCBI Official Full Name: angiotensin I converting enzyme, partial
• NCBI Official Synonym Full Names: angiotensin I converting enzyme
• NCBI Official Symbol: ACE
• NCBI Official Synonym Symbols: DCP; ICH; ACE1; DCP1; CD143; MVCD3
• NCBI Protein Information: angiotensin-converting enzyme; kininase II; peptidase P; CD143 antigen; testicular ECA; carboxycathepsin; dipeptidyl carboxypeptidase 1; dipeptidyl carboxypeptidase I; angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript
• UniProt Protein Name: Angiotensin-converting enzyme
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: ACE
• UniProt Synonym Gene Names: DCP; DCP1; ACE
• UniProt Entry Name: ACE_HUMAN

NCBI Description

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]

Uniprot Description

ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.15.1; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; lysosome; plasma membrane; integral to membrane; extracellular region; endosome; external side of plasma membrane

Molecular Function: peptidyl-dipeptidase activity; tripeptidyl-peptidase activity; zinc ion binding; carboxypeptidase activity; metallopeptidase activity; mitogen-activated protein kinase kinase binding; drug binding; actin binding; protein binding; bradykinin receptor binding; endopeptidase activity; exopeptidase activity; mitogen-activated protein kinase binding; chloride ion binding

Biological Process: mononuclear cell proliferation; regulation of vasodilation; angiotensin mediated regulation of renal output; neutrophil mediated immunity; regulation of angiotensin metabolic process; proteolysis; angiotensin maturation; arachidonic acid secretion; regulation of systemic arterial blood pressure by renin-angiotensin; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; regulation of smooth muscle cell migration; heart contraction; peptide catabolic process; regulation of blood pressure; beta-amyloid metabolic process; regulation of vasoconstriction; angiotensin catabolic process in blood; blood vessel remodeling; spermatogenesis; hormone catabolic process; kidney development

Disease: Renal Tubular Dysgenesis; Microvascular Complications Of Diabetes, Susceptibility To, 3; Alzheimer Disease; Hemorrhage, Intracerebral, Susceptibility To