Mouse 3-ketoacyl-CoA thiolase peroxisomal ELISA Kit | ACAA1 elisa kit

Mouse 3-ketoacyl-CoA thiolase peroxisomal (ACAA1) ELISA Kit

Cat. Num. AAA2604479

• Gene Names: ACAA1; ACAA; THIO; PTHIO
• Reactivity: Mouse
• Synonyms: 3-ketoacyl-CoA thiolase peroxisomal; Mouse 3-ketoacyl-CoA thiolase peroxisomal (ACAA1) ELISA Kit; ACAA1 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 424

• Samples: Mouse serum, plasma or cell culture supernatant and organizations
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACAA1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ACAA1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4501853
• NCBI GeneID: 30
• NCBI Accession #: NP_001598.1
• NCBI GenBank Nucleotide #: NM_001607.3
• Molecular Weight: 34,665 Da
• NCBI Official Full Name: 3-ketoacyl-CoA thiolase, peroxisomal isoform a
• NCBI Official Synonym Full Names: acetyl-CoA acyltransferase 1
• NCBI Official Symbol: ACAA1
• NCBI Official Synonym Symbols: ACAA; THIO; PTHIO
• NCBI Protein Information: 3-ketoacyl-CoA thiolase, peroxisomal
• UniProt Protein Name: 3-ketoacyl-CoA thiolase, peroxisomal
• UniProt Gene Name: ACAA1
• UniProt Synonym Gene Names: ACAA; PTHIO
• UniProt Entry Name: THIK_HUMAN

NCBI Description

This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

ACAA1: an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Protein type: EC 2.3.1.16; Lipid Metabolism - unsaturated fatty acid biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Acetyltransferase; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: intracellular membrane-bound organelle; membrane; peroxisomal matrix; peroxisome

Molecular Function: acetyl-CoA C-acyltransferase activity; palmitoyl-CoA oxidase activity; protein binding

Biological Process: bile acid metabolic process; cellular lipid metabolic process; fatty acid beta-oxidation; fatty acid beta-oxidation using acyl-CoA oxidase; unsaturated fatty acid metabolic process; very-long-chain fatty acid metabolic process

Disease: D-bifunctional Protein Deficiency