ABSCISIC ACID-INSENSITIVE 5 Recombinant Protein | ABI5 recombinant protein

Recombinant Arabidopsis thaliana Protein ABSCISIC ACID-INSENSITIVE 5

Cat. Num. AAA1354042

• Gene Names: ABI5; ABA INSENSITIVE 5; ABI5; F2H17.12; F2H17_12; GIA1; GROWTH-INSENSITIVITY TO ABA 1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: ABSCISIC ACID-INSENSITIVE 5; Recombinant Arabidopsis thaliana Protein ABSCISIC ACID-INSENSITIVE 5; Dc3 promoter-binding factor 1; AtDPBF1; Protein GROWTH-INSENSITIVITY TO ABA 1bZIP transcription factor 39; AtbZIP39; ABI5 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-442aa; Full Length
• Sequence: MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSLGRQSSIYSLTLDEFQHALCENGKNFGSMNMDEFLVSIWNAEENNNNQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPTFGEMTLEDFLVKAGVVREHPTNPKPNPNPNQNQNPSSVIPAAAQQQLYGVFQGTGDPSFPGQAMGVGDPSGYAKRTGGGGYQQAPPVQAGVCYGGGVGFGAGGQQMGMVGPLSPVSSDGLGHGQVDNIGGQYGVDMGGLRGRKRVVDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL
• Sequence Length: 442

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for ABI5 recombinant protein

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the 1 and 6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence

References

The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor.Finkelstein R.R., Lynch T.J.Plant Cell 12:599-609(2000) Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities.Kim S.Y., Ma J., Perret P., Li Z., Thomas T.L.Plant Physiol. 130:688-697(2002) Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.Nature 402:761-768(1999) The Arabidopsis Information Resource (TAIR) Arabidopsis ORF Clones.Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana.Lopez-Molina L., Chua N.H.Plant Cell Physiol. 41:541-547(2000) Physical interactions between ABA response loci of Arabidopsis.Nakamura S., Lynch T.J., Finkelstein R.R.Plant J. 26:627-635(2001) A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis.Lopez-Molina L., Mongrand S., Chua N.-H.Proc. Natl. Acad. Sci. U.S.A. 98:4782-4787(2001) The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis.Bensmihen S., Rippa S., Lambert G., Jublot D., Pautot V., Granier F., Giraudat J., Parcy F.Plant Cell 14:1391-1403(2002) Regulation of Arabidopsis thaliana Em genes role of ABI5.Carles C., Bies-Etheve N., Aspart L., Leon-Kloosterziel K.M., Koornneef M., Echeverria M., Delseny M.Plant J. 30:373-383(2002) ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination.Lopez-Molina L., Mongrand S., McLachlin D.T., Chait B.T., Chua N.-H.Plant J. 32:317-328(2002) Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response.Brocard I.M., Lynch T.J., Finkelstein R.R.Plant Physiol. 129:1533-1543(2002) Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid.Lu C., Han M.-H., Guevara-Garcia A., Fedoroff N.V.Proc. Natl. Acad. Sci. U.S.A. 99:15812-15817(2002) bZIP transcription factors in Arabidopsis.Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J., Tiedemann J., Kroj T., Parcy F.Trends Plant Sci. 7:106-111(2002) AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation.Lopez-Molina L., Mongrand S., Kinoshita N., Chua N.-H.Genes Dev. 17:410-418(2003) The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling.Smalle J., Kurepa J., Yang P., Emborg T.J., Babiychuk E., Kushnir S., Vierstra R.D.Plant Cell 15:965-980(2003) Three genes that affect sugar sensing (abscisic acid insensitive 4, abscisic acid insensitive 5, and constitutive triple response 1) are differentially regulated by glucose in Arabidopsis.Arroyo A., Bossi F., Finkelstein R.R., Leon P.Plant Physiol. 133:231-242(2003) Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence.Pourtau N., Mares M., Purdy S., Quentin N., Rueel A., Wingler A.Planta 219:765-772(2004) Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI) 5 and ABRE-BINDING FACTOR (ABF) 3.Finkelstein R.R., Gampala S.S., Lynch T.J., Thomas T.L., Rock C.D.Plant Mol. Biol. 59:253-267(2005) KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling.Stone S.L., Williams L.A., Farmer L.M., Vierstra R.D., Callis J.Plant Cell 18:3415-3428(2006) Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis.Nakashima K., Fujita Y., Katsura K., Maruyama K., Narusaka Y., Seki M., Shinozaki K., Yamaguchi-Shinozaki K.Plant Mol. Biol. 60:51-68(2006) Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis.Fujii H., Verslues P.E., Zhu J.-K.Plant Cell 19:485-494(2007) A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings.Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.Plant Mol. Biol. 67:643-658(2008) Integration of light and abscisic acid signaling during seed germination and early seedling development.Chen H., Zhang J., Neff M.M., Hong S.-W., Zhang H., Deng X.-W., Xiong L.Proc. Natl. Acad. Sci. U.S.A. 105:4495-4500(2008) Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling.Miura K., Lee J., Jin J.B., Yoo C.Y., Miura T., Hasegawa P.M.Proc. Natl. Acad. Sci. U.S.A. 106:5418-5423(2009) The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors.Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H., Zheng W., Sun J., Li C.Plant Cell 24:2898-2916(2012)

NCBI and Uniprot Product Information

• NCBI GI #: 18404091
• NCBI GeneID: 818199
• NCBI Accession #: NP_565840.1
• NCBI GenBank Nucleotide #: NM_129185.3
• UniProt Accession #: Q9SJN0
• Molecular Weight: 63 kDa
• NCBI Official Full Name: protein abscisic acid-insensitive 5
• NCBI Official Symbol: ABI5
• NCBI Official Synonym Symbols: ABA INSENSITIVE 5; ABI5; F2H17.12; F2H17_12; GIA1; GROWTH-INSENSITIVITY TO ABA 1
• NCBI Protein Information: protein abscisic acid-insensitive 5
• UniProt Protein Name: Protein ABSCISIC ACID-INSENSITIVE 5
• Protein Family: ABSCISIC ACID-INSENSITIVE 5-like protein
• UniProt Gene Name: ABI5
• UniProt Synonym Gene Names: BZIP39; DPBF1; GIA1; NEM1; AtDPBF1; AtbZIP39
• UniProt Entry Name: ABI5_ARATH

NCBI Description

Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages.

Uniprot Description

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.