ABCG2 / ATP-Binding Cassette Sub-Family G Member 2 Recombinant Protein | ABCG2 recombinant protein

Human ABCG2 / ATP-Binding Cassette Sub-Family G Member 2 Recombinant Protein

• Gene Names: ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: ABCG2 / ATP-Binding Cassette Sub-Family G Member 2; Human ABCG2 / ATP-Binding Cassette Sub-Family G Member 2 Recombinant Protein; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; CD338; ABCP; BCRP; BCRP1; MXR; ABCG2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 655

• Applicable Applications for ABCG2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Monomer under reducing conditions, the minimal functional units is a homodimer; disulfide-linked, but the major oligomeric form in plasma membranes is a homotetramer with possibility of higher order oligomerization up to homododecamers.
• Entry Function: High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 67462103
• NCBI GeneID: 9429
• UniProt Accession #: Q9UNQ0
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 2 (Junior blood group)
• NCBI Official Symbol: ABCG2
• NCBI Official Synonym Symbols: MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• NCBI Protein Information: ATP-binding cassette sub-family G member 2
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG2
• UniProt Synonym Gene Names: ABCP; BCRP; BCRP1; MXR
• UniProt Entry Name: ABCG2_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

ABCG2: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123. Monomer or homodimer; disulfide-linked. Up-regulated in brain tumors. Highly expressed in placenta. Low expression in small intestine, liver and colon. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, iron; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: nucleoplasm; mitochondrial membrane; integral to membrane; plasma membrane; nucleus

Molecular Function: protein binding; protein homodimerization activity; ATPase activity, coupled to transmembrane movement of substances; transporter activity; heme transporter activity; xenobiotic-transporting ATPase activity; ATP binding

Biological Process: response to drug; heme transport; urate metabolic process; transport; cellular iron ion homeostasis; xenobiotic transport; multidrug transport; transmembrane transport

Disease: Blood Group, Junior System; Uric Acid Concentration, Serum, Quantitative Trait Locus 1