Rat ABCG1 ELISA Kit | ABC G1 elisa kit

Rat ABCG1 (ATP Binding Cassette Transporter G1) ELISA Kit

Cat. Num. AAA2503657

• Gene Names: ABCG1; ABC8; WHITE1
• Reactivity: Rat
• Synonyms: ABCG1; Rat ABCG1 (ATP Binding Cassette Transporter G1) ELISA Kit; ABC G1 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat ABCG1. No significant cross-reactivity or interference between Rat ABCG1 and analogues was observed.
• Sequence Length: 663

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ABCG1 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABC G1 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ABCG1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ABCG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ABCG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ABCG1. You can calculate the concentration of ABCG1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 46592978
• NCBI GeneID: 9619
• NCBI Accession #: NP_997512.1
• NCBI GenBank Nucleotide #: NM_207629.1
• UniProt Accession #: P45844; Q86SU8; Q96L76; Q9BXK6; Q9BXK7; Q9BXK8; Q9BXK9; Q9BXL0; Q9BXL1; Q9BXL2; Q9BXL3; Q9BXL4
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 1 isoform 7
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1; ABC transporter 8; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; homolog of Drosophila white; white protein homolog (ATP-binding cassette transporter 8)
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, ABC family; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; recycling endosome; intracellular membrane-bound organelle; mitochondrion; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; toxin transporter activity; cholesterol binding; phospholipid binding; sterol-transporting ATPase activity; ADP binding; ATP binding

Biological Process: cholesterol metabolic process; response to high density lipoprotein stimulus; detection of hormone stimulus; positive regulation of cholesterol biosynthetic process; intracellular cholesterol transport; lipoprotein metabolic process; cholesterol efflux; response to lipid; response to organic substance; amyloid precursor protein catabolic process; cholesterol homeostasis; reverse cholesterol transport; regulation of transcription, DNA-dependent; phospholipid efflux; transmembrane transport; phospholipid homeostasis