Chicken Anti-Apolipoprotein Antibodies ELISA Kit | AAHA elisa kit

Chicken Anti-Apolipoprotein Antibodies ELISA Kit

Cat. Num. AAA740202

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Chicken
• Synonyms: Anti-Apolipoprotein Antibodies; Chicken Anti-Apolipoprotein Antibodies ELISA Kit; AAHA elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 u/L.
• Intended Uses: This AAHA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AAHA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AAHA elisa kit

Principle of the Assay: AAHA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AAHA antibody and an AAHA-HRP conjugate. The assay sample and buffer are incubated together with AAHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AAHA concentration since AAHA from samples and AAHA-HRP conjugate compete for the anti-AAHA antibody binding site. Since the number of sites is limited, as more sites are occupied by AAHA from the sample, fewer sites are left to bind AAHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AAHA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AAHA elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 105990532
• NCBI GeneID: 338
• NCBI Accession #: NP_000375.2
• NCBI GenBank Nucleotide #: NM_000384.2
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted; Carrier; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol; actin cytoskeleton; chylomicron; cell soma; cytoplasm; plasma membrane; endosome membrane

Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding

Biological Process: lipoprotein catabolic process; phototransduction, visible light; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; receptor-mediated endocytosis; nervous system development; cholesterol metabolic process; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; lipoprotein metabolic process; cholesterol efflux; sperm motility; cholesterol homeostasis; fertilization; lipoprotein biosynthetic process; lipoprotein transport; artery morphogenesis; spermatogenesis; triacylglycerol mobilization; blood coagulation; leukocyte migration

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1