Alpha-1-B-Glycoprotein (a1BG) Recombinant Protein | a1BG recombinant protein
Recombinant Alpha-1-B-Glycoprotein (a1BG)
Gene Names
A1bg; C44
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Alpha-1-B-Glycoprotein (a1BG); Recombinant Alpha-1-B-Glycoprotein (a1BG); a1BG recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGS-Q LPPSLCLMGS YLTIYPQKTH ETLACKAPRN AAEFQLRQGE RVLNIQGFSP TRDATIYYVN LKELDNQSPF TCRYRMHKYM HVWSEDSKPV ELMWSDEKLP APVLTAEPSS HNLEPGSTVQ LRCTAHKAGL RFGLQRQGKP DLVVVQMLNS SGTEAVFELH NISTIDSGNY SCIYMEQAPP FSGSASSEPL ELRIN
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGS-Q LPPSLCLMGS YLTIYPQKTH ETLACKAPRN AAEFQLRQGE RVLNIQGFSP TRDATIYYVN LKELDNQSPF TCRYRMHKYM HVWSEDSKPV ELMWSDEKLP APVLTAEPSS HNLEPGSTVQ LRCTAHKAGL RFGLQRQGKP DLVVVQMLNS SGTEAVFELH NISTIDSGNY SCIYMEQAPP FSGSASSEPL ELRIN
Sequence Length
513
Applicable Applications for a1BG recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Gln220~Asn415 (Accession # Q9EPH1) with two N-terminal Tags, His-tag and S-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27.5kDa
NCBI Official Full Name
alpha-1B-glycoprotein
NCBI Official Synonym Full Names
alpha-1-B glycoprotein
NCBI Official Symbol
A1bg
NCBI Official Synonym Symbols
C44
NCBI Protein Information
alpha-1B-glycoprotein; liver regeneration-related protein 1
UniProt Protein Name
Alpha-1B-glycoprotein
Protein Family
UniProt Gene Name
A1bg
UniProt Entry Name
A1BG_RAT
NCBI Description
may have a role in the immediate-early response phase of liver regeneration [RGD, Feb 2006]
Uniprot Description
Subunit structure: Interacts with CRISP3
By similarity.
Subcellular location: Secreted
By similarity UniProtKB P04217.
Tissue specificity: Isoform 1 is expressed in normal liver. Isoform 2 is expressed in the regenerating liver after partial hepatectomy and at very low levels in the normal lung, brain and testis. Ref.1 Ref.3
Developmental stage: In females, isoform 1 is expressed at day 35 with higher levels detected at day 56. Isoform 1 is not detected in males of any age. Ref.1
Induction: Isoform 1 is up-regulated by continuous exposure to growth hormone. Ref.1
Sequence similarities: Contains 5 Ig-like V-type (immunoglobulin-like) domains.
Sequence caution: The sequence AAF68963.1 differs from that shown. Reason: Erroneous initiation.
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Product Notes
The a1BG a1bg (Catalog #AAA2010688) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha-1-B-Glycoprotein (a1BG) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the a1BG a1bg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS -Q LPPSLCLMGS YLTIYPQKTH ETLACKAPRN AAEFQLRQGE RVLNIQGFSP TRDATIYYVN LKELDNQSPF TCRYRMHKYM HVWSEDSKPV ELMWSDEKLP APVLTAEPSS HNLEPGSTVQ LRCTAHKAGL RFGLQRQGKP DLVVVQMLNS SGTEAVFELH NISTIDSGNY SCIYMEQAPP FSGSASSEPL ELRIN. It is sometimes possible for the material contained within the vial of "Alpha-1-B-Glycoprotein (a1BG), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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