His-Tag Protein (Ni-NTA) Purification Kit | Ni-NTA purification kit
His-Tag Protein Purification Kit (Ni-NTA)
Synonyms
His-Tag Protein (Ni-NTA); His-Tag Protein Purification Kit (Ni-NTA); Ni-NTA; His resin; Ni-NTA resin; His tag resin; Ni-NTA purification kit
Tag
His Tag
Features & Benefits
High capacity: More than 50mg of 6xHis-tagged protein per milliliter of resin.
Versatile: For purification under native or denaturing conditions.
Cost-effective: No performance decrease after at least five repeated uses of the same batch of resin.
Flexible: available in multiple formats including bulk resin, spin columns and complete kits.
Easy to use: Pre-formulated buffers available for kit formats.
His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90um beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid: NTA has been coupled. The chelating group has then been charged with nickel ions: Ni2+ Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Versatile: For purification under native or denaturing conditions.
Cost-effective: No performance decrease after at least five repeated uses of the same batch of resin.
Flexible: available in multiple formats including bulk resin, spin columns and complete kits.
Easy to use: Pre-formulated buffers available for kit formats.
His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90um beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid: NTA has been coupled. The chelating group has then been charged with nickel ions: Ni2+ Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Kit Components
His-Tag Purification Nickel Column
Phosphate-Buffered Saline (PBS,10X)
Imidazole (2M)
Phosphate-Buffered Saline (PBS,10X)
Imidazole (2M)
Preparation and Storage
Stable for one year at 2-8 degree C from date of shipment. Do not freeze.
Shipping||Blue ice
Shipping||Blue ice
Related Product Information for Ni-NTA purification kit
Description: His Tagged Protein Purification Protein Purification Kit
Background: His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The His-Tag Purification resin is also available as prepacked spin column and kit formats.
Background: His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The His-Tag Purification resin is also available as prepacked spin column and kit formats.
Product Categories/Family for Ni-NTA purification kit
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Product Notes
The Ni-NTA (Catalog #AAA9719239) is a Purification Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "His-Tag Protein (Ni-NTA), Purification Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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