FITC Labeling Kit
LinKine FITC Labeling Kit (Optimized for samples with molecular weight of 6 KD to 20 KD)
Synonyms
FITC; LinKine FITC Labeling Kit (Optimized for samples with molecular weight of 6 KD to 20 KD); Fluorescein isothiocyanate; LinKine FITC Labeling Kit; FITC labeling kit
Tag
FITC
Conjugation
FITC
Features & Benefits
Easy-Ready for Labeling to antibodies and other proteins.
Optimized kit procedure-following the standard protocol results in antibodies with excellent dye: protein ratios for optimum activity and fluorescence.
Single-use fluors-kits contain single-use vials of reagent.
Efficient purification-kits include purification columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery.
LinKine FITC Labeling Kit: Optimized for samples with molecular weight of 6KD to 20KD is designed for preparing FITC conjugates directly from proteins, peptides, and other ligands that contain a free amino group.
Optimized kit procedure-following the standard protocol results in antibodies with excellent dye: protein ratios for optimum activity and fluorescence.
Single-use fluors-kits contain single-use vials of reagent.
Efficient purification-kits include purification columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery.
LinKine FITC Labeling Kit: Optimized for samples with molecular weight of 6KD to 20KD is designed for preparing FITC conjugates directly from proteins, peptides, and other ligands that contain a free amino group.
Kit Components
Activated FITC solution
FITC labeling solution
Purification column (0.5 mL, 3KD)
FITC labeling solution
Purification column (0.5 mL, 3KD)
Usage Notes
The initial concentration of the sample to be labelled should not less than 2mg/ml.
Please note that components that containing amino are not allowed in the buffer, which will affect the coupling effects.
A small amount of BSA will not affect the results, and PBS is recommended to use as the required buffer.
Please note that components that containing amino are not allowed in the buffer, which will affect the coupling effects.
A small amount of BSA will not affect the results, and PBS is recommended to use as the required buffer.
Preparation and Storage
Store kit components at -20 degree C for 6 months.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for FITC labeling kit
Description: LinKine FITC Labeling Kit (Optimized for samples with molecular weight of 6 KD to 20 KD) is designed for preparing FITC conjugates directly from proteins, peptides, and other ligands that contain a free amino group.
Background: Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. FITC has excitation and emission spectrum peak wavelengths of approximately 492nm/520nm, giving it a green color.
Background: Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. FITC has excitation and emission spectrum peak wavelengths of approximately 492nm/520nm, giving it a green color.
Product Categories/Family for FITC labeling kit
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Product Notes
The FITC (Catalog #AAA9719233) is a Labeling Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FITC, Labeling Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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