Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Glyoxalase domain-containing protein 4 (GLOD4) Standard Curve.)
Mouse Glyoxalase Domain-Containing Protein 4 (GLOD4) ELISA Kit | GLOD4 elisa kit
Mouse Glyoxalase Domain-Containing Protein 4 (GLOD4) ELISA Kit
Gene Names
Glod4; C81254; 1700082G03Rik; 2700085E05Rik
Reactivity
Mouse
Synonyms
Glyoxalase Domain-Containing Protein 4 (GLOD4); Mouse Glyoxalase Domain-Containing Protein 4 (GLOD4) ELISA Kit; GLOD4; C17orf25; CGI-150; HC71; GLOD4 elisa kit
Reactivity
Mouse
Specificity
Mouse Glyoxalase domain-containing protein 4 (GLOD4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GLOD4. No significant cross-reactivity or interference between Mouse GLOD4 and analogues was observed.
Sequence Length
298
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Glyoxalase domain-containing protein 4 (GLOD4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Glyoxalase domain-containing protein 4 (GLOD4) Standard Curve.)
Related Product Information for GLOD4 elisa kit
Description: This Mouse Glyoxalase domain-containing protein 4 (GLOD4) ELISA Kit employs a two-site sandwich ELISA to quantitate GLOD4 in samples. An antibody specific for GLOD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLOD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLOD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLOD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: C17orf25 is divided into 10 exons and 9 introns, spanning 23 kb of genomic DNA. The mRNA expression of C17orf25 was decreased in hepatocellular carcinoma samples as compared to adjacent noncancerous liver tissues from the same patients. The transfection of C17orf25 into the hepatocellular carcinoma cell SMMC7721 and overexpression could inhibit the cell growth. The above results indicate that C17orf25 is a novel human gene, and the cloning and preliminary characterization of C17orf25 is a prerequisite for further functional analysis of this novel gene in human hepatocellular carcinoma.The human C17orf25 gene is one of thirteen genes cloned from a region displaying a high score of loss of heterozygosity within chromosome 17p13.3 in human hepatocellular carcinoma in China.
Background: C17orf25 is divided into 10 exons and 9 introns, spanning 23 kb of genomic DNA. The mRNA expression of C17orf25 was decreased in hepatocellular carcinoma samples as compared to adjacent noncancerous liver tissues from the same patients. The transfection of C17orf25 into the hepatocellular carcinoma cell SMMC7721 and overexpression could inhibit the cell growth. The above results indicate that C17orf25 is a novel human gene, and the cloning and preliminary characterization of C17orf25 is a prerequisite for further functional analysis of this novel gene in human hepatocellular carcinoma.The human C17orf25 gene is one of thirteen genes cloned from a region displaying a high score of loss of heterozygosity within chromosome 17p13.3 in human hepatocellular carcinoma in China.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glyoxalase domain-containing protein 4 isoform 1
NCBI Official Synonym Full Names
glyoxalase domain containing 4
NCBI Official Symbol
Glod4
NCBI Official Synonym Symbols
C81254; 1700082G03Rik; 2700085E05Rik
NCBI Protein Information
glyoxalase domain-containing protein 4
UniProt Protein Name
Glyoxalase domain-containing protein 4
Protein Family
UniProt Gene Name
Glod4
UniProt Entry Name
GLOD4_MOUSE
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Product Notes
The Mouse GLOD4 glod4 (Catalog #AAA9718092) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718092 ELISA Kit recognizes Mouse GLOD4. It is sometimes possible for the material contained within the vial of "Glyoxalase Domain-Containing Protein 4 (GLOD4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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