Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) Standard Curve.)
Mouse Peptidyl-prolyl cis-Trans Isomerase C (PPIC) ELISA Kit | PPIC elisa kit
Mouse Peptidyl-prolyl cis-Trans Isomerase C (PPIC) ELISA Kit
Gene Names
Ppic; CyP-20c
Reactivity
Mouse
Synonyms
Peptidyl-prolyl cis-Trans Isomerase C (PPIC); Mouse Peptidyl-prolyl cis-Trans Isomerase C (PPIC) ELISA Kit; PPIC; CYPC; MGC3673; PPIase C; cyclophilin C; parvulin; peptidyl-prolyl cis-trans isomerase C; peptidylprolyl isomerase C; rotamase C; PPIC elisa kit
Reactivity
Mouse
Specificity
Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPIC. No significant cross-reactivity or interference between Mouse PPIC and analogues was observed.
Sequence Length
212
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) Standard Curve.)
Related Product Information for PPIC elisa kit
Description: This Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit employs a two-site sandwich ELISA to quantitate PPIC in samples. An antibody specific for PPIC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPIC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPIC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPIC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peptidyl-prolyl cis-trans isomerase C is a member of the peptidyl-prolyl cis-trans isomerase (PPIase)) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. Friedman and Weissman (1991) cloned cyclophilin C from a cDNA library prepared from a murine bone marrow-derived stromal cell line. Their initial interest in the cDNA was based on its high level of homology with all known cyclophilins. The tissue distribution made it a candidate for the mediator of the immunosuppressive and nephrotoxic actions of cyclosporin A, as it is expressed most highly in the kidney and can be detected in activated T cells.
Background: Peptidyl-prolyl cis-trans isomerase C is a member of the peptidyl-prolyl cis-trans isomerase (PPIase)) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. Friedman and Weissman (1991) cloned cyclophilin C from a cDNA library prepared from a murine bone marrow-derived stromal cell line. Their initial interest in the cDNA was based on its high level of homology with all known cyclophilins. The tissue distribution made it a candidate for the mediator of the immunosuppressive and nephrotoxic actions of cyclosporin A, as it is expressed most highly in the kidney and can be detected in activated T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase C
NCBI Official Synonym Full Names
peptidylprolyl isomerase C
NCBI Official Symbol
Ppic
NCBI Official Synonym Symbols
CyP-20c
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase C
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase C
Protein Family
UniProt Gene Name
Ppic
UniProt Synonym Gene Names
Cypc; PPIase C
UniProt Entry Name
PPIC_MOUSE
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Product Notes
The Mouse PPIC ppic (Catalog #AAA9718046) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718046 ELISA Kit recognizes Mouse PPIC. It is sometimes possible for the material contained within the vial of "Peptidyl-prolyl cis-Trans Isomerase C (PPIC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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