Mouse Nucleolin (NCL) ELISA Kit | NCL elisa kit

Mouse Nucleolin (NCL) ELISA Kit

Cat. Num. AAA9717414

• Gene Names: Ncl; C23; Nucl; D0Nds28; D1Nds28; B530004O11Rik
• Reactivity: Mouse
• Synonyms: Nucleolin (NCL); Mouse Nucleolin (NCL) ELISA Kit; NCL; C23; FLJ45706; FLJ59041; NCL elisa kit

• Reactivity: Mouse
• Specificity: Mouse Nucleolin (NCL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCL. No significant cross-reactivity or interference between Mouse NCL and analogues was observed.
• Sequence Length: 707

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Nucleolin (NCL) Standard Curve.)

Related Product Information for NCL elisa kit

Description: This Mouse Nucleolin (NCL) ELISA Kit employs a two-site sandwich ELISA to quantitate NCL in samples. An antibody specific for NCL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

NCBI and Uniprot Product Information

• NCBI GI #: 84875537
• NCBI GeneID: 17975
• NCBI Accession #: NP_035010.3
• NCBI GenBank Nucleotide #: NP_035010.3
• UniProt Accession #: P09405
• NCBI Official Full Name: nucleolin
• NCBI Official Synonym Full Names: nucleolin
• NCBI Official Symbol: Ncl
• NCBI Official Synonym Symbols: C23; Nucl; D0Nds28; D1Nds28; B530004O11Rik
• NCBI Protein Information: nucleolin
• UniProt Protein Name: Nucleolin
• Protein Family: Nucleolin
• UniProt Gene Name: Ncl
• UniProt Synonym Gene Names: Nuc

Uniprot Description

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats ().