Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Periplakin (PPL) Standard Curve.)
Mouse Periplakin (PPL) ELISA Kit | PPL elisa kit
Mouse Periplakin (PPL) ELISA Kit
Gene Names
Ppl; AW553870
Reactivity
Mouse
Synonyms
Periplakin (PPL); Mouse Periplakin (PPL) ELISA Kit; PPL; KIAA0568; MGC134872; 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope; PPL elisa kit
Reactivity
Mouse
Specificity
Mouse Periplakin (PPL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPL. No significant cross-reactivity or interference between Mouse PPL and analogues was observed.
Sequence Length
1754
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Periplakin (PPL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Periplakin (PPL) Standard Curve.)
Related Product Information for PPL elisa kit
Description: This Mouse Periplakin (PPL) ELISA Kit employs a two-site sandwich ELISA to quantitate PPL in samples. An antibody specific for PPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Periplakin is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.The deduced 1,666-amino acid protein has a calculated molecular mass of about 205 kD. PPL shares extensive sequence homology with other proteins of the plakin family, with highest identities found in the N- and C-terminal domains.
Background: Periplakin is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.The deduced 1,666-amino acid protein has a calculated molecular mass of about 205 kD. PPL shares extensive sequence homology with other proteins of the plakin family, with highest identities found in the N- and C-terminal domains.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
periplakin
NCBI Official Synonym Full Names
periplakin
NCBI Official Symbol
Ppl
NCBI Official Synonym Symbols
AW553870
NCBI Protein Information
periplakin
UniProt Protein Name
Periplakin
Protein Family
UniProt Gene Name
Ppl
Uniprot Description
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Research Articles on PPL
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Product Notes
The Mouse PPL ppl (Catalog #AAA9717314) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717314 ELISA Kit recognizes Mouse PPL. It is sometimes possible for the material contained within the vial of "Periplakin (PPL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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