Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Replication initiator 1 (REPIN1) Standard Curve.)
Mouse Replication Initiator 1 (REPIN1) ELISA Kit | REPIN1 elisa kit
Mouse Replication Initiator 1 (REPIN1) ELISA Kit
Gene Names
Repin1; Ap4; Zfp464; Zfp-464; AI425994; E430037F08Rik
Reactivity
Mouse
Synonyms
Replication Initiator 1 (REPIN1); Mouse Replication Initiator 1 (REPIN1) ELISA Kit; REPIN1; AP4; RIP60; ZNF464; Zfp464; H_DJ0584D14.12; replication initiation region protein (60kD); zinc finger protein 464 (RIP60); zinc finger protein AP4; zinc finger proten AP4; REPIN1 elisa kit
Reactivity
Mouse
Specificity
Mouse Replication initiator 1 (REPIN1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse REPIN1. No significant cross-reactivity or interference between Mouse REPIN1 and analogues was observed.
Sequence Length
598
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Replication initiator 1 (REPIN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Replication initiator 1 (REPIN1) Standard Curve.)
Related Product Information for REPIN1 elisa kit
Description: This Mouse Replication initiator 1 (REPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate REPIN1 in samples. An antibody specific for REPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Replication initiator 1 is a protein encoded by the REPIN1 gene. Homodimers of RIP60 (replication initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro. RIP60 contains 15 C(2)H(2)zinc finger (ZF) DNA binding motifs organized in three clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15). A proline-rich region is located between hands Z2 and Z3. Gel mobility shift and DNase I footprinting experiments show hands Z1 and Z2 independently bind the oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds DNA, but displays no specificity for RIP60 sites.
Background: Replication initiator 1 is a protein encoded by the REPIN1 gene. Homodimers of RIP60 (replication initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro. RIP60 contains 15 C(2)H(2)zinc finger (ZF) DNA binding motifs organized in three clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15). A proline-rich region is located between hands Z2 and Z3. Gel mobility shift and DNase I footprinting experiments show hands Z1 and Z2 independently bind the oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds DNA, but displays no specificity for RIP60 sites.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
replication initiator 1 isoform a
NCBI Official Synonym Full Names
replication initiator 1
NCBI Official Symbol
Repin1
NCBI Official Synonym Symbols
Ap4; Zfp464; Zfp-464; AI425994; E430037F08Rik
NCBI Protein Information
replication initiator 1
UniProt Protein Name
Replication initiator 1
Protein Family
UniProt Gene Name
Repin1
UniProt Synonym Gene Names
Zfp464; Zfp-464
Uniprot Description
Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending ().
Research Articles on REPIN1
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Product Notes
The Mouse REPIN1 repin1 (Catalog #AAA9717115) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717115 ELISA Kit recognizes Mouse REPIN1. It is sometimes possible for the material contained within the vial of "Replication Initiator 1 (REPIN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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