Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protein 97 (TMEM97/MAC30) Standard Curve.)
Mouse Transmembrane Protein 97 (TMEM97/MAC30) ELISA Kit | TMEM97/MAC30 elisa kit
Mouse Transmembrane Protein 97 (TMEM97/MAC30) ELISA Kit
Gene Names
Tmem97; AI115531; AL022956; D11Bhm182e; 1810014L12Rik
Reactivity
Mouse
Synonyms
Transmembrane Protein 97 (TMEM97/MAC30); Mouse Transmembrane Protein 97 (TMEM97/MAC30) ELISA Kit; TMEM97/MAC30; MAC30; TMEM97/MAC30 elisa kit
Reactivity
Mouse
Specificity
Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM97/MAC30. No significant cross-reactivity or interference between Mouse TMEM97/MAC30 and analogues was observed.
Sequence Length
176
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protein 97 (TMEM97/MAC30) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protein 97 (TMEM97/MAC30) Standard Curve.)
Related Product Information for TMEM97/MAC30 elisa kit
Description: This Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM97/MAC30 in samples. An antibody specific for TMEM97/MAC30 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM97/MAC30 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM97/MAC30 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM97/MAC30 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels.MAC30 expression in all normal human tissues examined, with highest expression in testis and stomach and lowest expression in thyroid, thymus, and adipose tissue.In situ hybridization detected MAC30 in normal pancreatic acini, islets, and large ducts. Immunohistochemical analysis showed MAC30 protein in the cytoplasm of peripheral pancreatic islet cells, in endothelial and smooth muscle cells of blood vessels, and in nerves, monocytes, and fibroblasts. In esophagus, MAC30 was detected in cytoplasm of mucosal cells, particularly in the superficial and intermediate epithelial layers. Gastric submucosa and muscularis and normal colonic epithelium exhibited staining patterns similar to that of esophagus.
Background: TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels.MAC30 expression in all normal human tissues examined, with highest expression in testis and stomach and lowest expression in thyroid, thymus, and adipose tissue.In situ hybridization detected MAC30 in normal pancreatic acini, islets, and large ducts. Immunohistochemical analysis showed MAC30 protein in the cytoplasm of peripheral pancreatic islet cells, in endothelial and smooth muscle cells of blood vessels, and in nerves, monocytes, and fibroblasts. In esophagus, MAC30 was detected in cytoplasm of mucosal cells, particularly in the superficial and intermediate epithelial layers. Gastric submucosa and muscularis and normal colonic epithelium exhibited staining patterns similar to that of esophagus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sigma intracellular receptor 2
NCBI Official Synonym Full Names
transmembrane protein 97
NCBI Official Symbol
Tmem97
NCBI Official Synonym Symbols
AI115531; AL022956; D11Bhm182e; 1810014L12Rik
NCBI Protein Information
sigma intracellular receptor 2
UniProt Protein Name
Transmembrane protein 97
UniProt Gene Name
Tmem97
UniProt Entry Name
TMM97_MOUSE
Uniprot Description
TMEM97: Plays a role as a regulator of cellular cholesterol homeostasis. Belongs to the TMEM97 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: nuclear membrane; rough endoplasmic reticulum; membrane; lysosome; endoplasmic reticulum; plasma membrane; integral to membrane; nucleus
Biological Process: cholesterol homeostasis
Research Articles on TMEM97/MAC30
Similar Products
Product Notes
The Mouse TMEM97/MAC30 tmem97 (Catalog #AAA9716772) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716772 ELISA Kit recognizes Mouse TMEM97/MAC30. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 97 (TMEM97/MAC30), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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