Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TNFAIP3-interacting protein 1 (TNIP1) Standard Curve.)
Mouse TNFAIP3-Interacting Protein 1 (TNIP1) ELISA Kit | TNIP1 elisa kit
Mouse TNFAIP3-Interacting Protein 1 (TNIP1) ELISA Kit
Gene Names
Tnip1; Nef; VAN; ABIN; Naf1; ABIN1; ABIN-1; AU018810
Reactivity
Mouse
Synonyms
TNFAIP3-Interacting Protein 1 (TNIP1); Mouse TNFAIP3-Interacting Protein 1 (TNIP1) ELISA Kit; TNIP1; ABIN-1; KIAA0113; NAF1; VAN; Nef-associated factor 1 SNP; virion-associated nuclear-shuttling protein; TNIP1 elisa kit
Reactivity
Mouse
Specificity
Mouse TNFAIP3-interacting protein 1 (TNIP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNIP1. No significant cross-reactivity or interference between Mouse TNIP1 and analogues was observed.
Sequence Length
594
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TNFAIP3-interacting protein 1 (TNIP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TNFAIP3-interacting protein 1 (TNIP1) Standard Curve.)
Related Product Information for TNIP1 elisa kit
Description: This Mouse TNFAIP3-interacting protein 1 (TNIP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNIP1 in samples. An antibody specific for TNIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Using a yeast 2-hybrid screen to identify proteins that interact with the human immunodeficiency virus (HIV)-1 Nef protein, Fukushi et al. (1999) isolated TNIP1, which they designated NAF1, from an activated leukocyte cDNA library. They obtained full-length NAF1 cDNAs by 5-prime and 3-prime RACE. Fukushi et al. (1999) identified 2 alternatively spliced isoforms of NAF1, NAF1-alpha and NAF1-beta, that differ only in their C-terminal amino acids. NAF1-alpha contains 636 amino acids, and NAF1-beta contains 640 amino acids. Both isoforms have 4 coiled-coil domains and a proline-rich C terminus. Northern blot analysis detected a 2.8-kb transcript in all tissues tested, with strong expression in peripheral blood lymphocytes, spleen, and skeletal muscle, and weak expression in brain.
Background: Using a yeast 2-hybrid screen to identify proteins that interact with the human immunodeficiency virus (HIV)-1 Nef protein, Fukushi et al. (1999) isolated TNIP1, which they designated NAF1, from an activated leukocyte cDNA library. They obtained full-length NAF1 cDNAs by 5-prime and 3-prime RACE. Fukushi et al. (1999) identified 2 alternatively spliced isoforms of NAF1, NAF1-alpha and NAF1-beta, that differ only in their C-terminal amino acids. NAF1-alpha contains 636 amino acids, and NAF1-beta contains 640 amino acids. Both isoforms have 4 coiled-coil domains and a proline-rich C terminus. Northern blot analysis detected a 2.8-kb transcript in all tissues tested, with strong expression in peripheral blood lymphocytes, spleen, and skeletal muscle, and weak expression in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TNFAIP3-interacting protein 1 isoform 2
NCBI Official Synonym Full Names
TNFAIP3 interacting protein 1
NCBI Official Symbol
Tnip1
NCBI Official Synonym Symbols
Nef; VAN; ABIN; Naf1; ABIN1; ABIN-1; AU018810
NCBI Protein Information
TNFAIP3-interacting protein 1
UniProt Protein Name
TNFAIP3-interacting protein 1
Protein Family
UniProt Gene Name
Tnip1
UniProt Synonym Gene Names
Abin; Naf1; ABIN; ABIN-1; Naf1; VAN; mVAN
UniProt Entry Name
TNIP1_MOUSE
Research Articles on TNIP1
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Product Notes
The Mouse TNIP1 tnip1 (Catalog #AAA9716721) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716721 ELISA Kit recognizes Mouse TNIP1. It is sometimes possible for the material contained within the vial of "TNFAIP3-Interacting Protein 1 (TNIP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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