Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human L-Lactate Dehydrogenase (L-LDH) Standard Curve.)
Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit | L-LDH elisa kit
Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit
Reactivity
Human
Synonyms
L-Lactate Dehydrogenase (L-LDH); Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit; L-LDH elisa kit
Reactivity
Human
Specificity
Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human L-LDH. No significant cross-reactivity or interference between Human L-LDH and analogues was observed.
Sequence Length
310
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human L-Lactate Dehydrogenase (L-LDH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human L-Lactate Dehydrogenase (L-LDH) Standard Curve.)
Related Product Information for L-LDH elisa kit
Description: This Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit employs a two-site sandwich ELISA to quantitate L-LDH in samples. An antibody specific for L-LDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any L-LDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L-LDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L-LDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. LDH exist in four distinct enzyme classes. This article is specifically about the NAD(P)-dependent L-lactate dehydrogenase. Other LDHs act on D-lactate and/or are dependent on cytochrome c: D-lactate dehydrogenase (cytochrome)) and L-lactate (L-lactate dehydrogenase (cytochrome)). LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.
Background: Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. LDH exist in four distinct enzyme classes. This article is specifically about the NAD(P)-dependent L-lactate dehydrogenase. Other LDHs act on D-lactate and/or are dependent on cytochrome c: D-lactate dehydrogenase (cytochrome)) and L-lactate (L-lactate dehydrogenase (cytochrome)). LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
L-lactate dehydrogenase
Similar Products
Product Notes
The Human L-LDH (Catalog #AAA9716210) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716210 ELISA Kit recognizes Human L-LDH. It is sometimes possible for the material contained within the vial of "L-Lactate Dehydrogenase (L-LDH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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