Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cystin-1 (CYS1) Standard Curve.)
Human Cystin-1 (CYS1) ELISA Kit | CYS1 elisa kit
Human Cystin-1 (CYS1) ELISA Kit
Reactivity
Human
Synonyms
Cystin-1 (CYS1); Human Cystin-1 (CYS1) ELISA Kit; CYS1; FLJ33980; CYS1 elisa kit
Reactivity
Human
Specificity
Human Cystin-1 (CYS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYS1. No significant cross-reactivity or interference between Human CYS1 and analogues was observed.
Sequence Length
158
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cystin-1 (CYS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cystin-1 (CYS1) Standard Curve.)
Related Product Information for CYS1 elisa kit
Description: This Human Cystin-1 (CYS1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYS1 in samples. An antibody specific for CYS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Cystin 1 Expressed at high levels in the kidney and pancreas. Moderate expression seen in the skeletal muscle, liver and heart. A weak expression seen in the brain, lung, uterus, prostate, testis, small intestine and colonVarious strains of mice that are homozygous for the cpk gene display renal pathology similar to that seen in human ARPKD. The PKD progresses to renal insufficiency, azotemia, and ultimately a uremic death by approximately 3 wk of age. The BALB/c-cpk/cpk murine model displays renal as well as extrarenal pathology similar to that found in human ARPKD. Renal mRNA expression of EGF is diminished in this mouse model. EGF treatment did not prevent renal failure but ameliorated pathologic changes in the kidney and the biliary ducts of the BALB/c-cpk/cpk mouse..
Background: Cystin 1 Expressed at high levels in the kidney and pancreas. Moderate expression seen in the skeletal muscle, liver and heart. A weak expression seen in the brain, lung, uterus, prostate, testis, small intestine and colonVarious strains of mice that are homozygous for the cpk gene display renal pathology similar to that seen in human ARPKD. The PKD progresses to renal insufficiency, azotemia, and ultimately a uremic death by approximately 3 wk of age. The BALB/c-cpk/cpk murine model displays renal as well as extrarenal pathology similar to that found in human ARPKD. Renal mRNA expression of EGF is diminished in this mouse model. EGF treatment did not prevent renal failure but ameliorated pathologic changes in the kidney and the biliary ducts of the BALB/c-cpk/cpk mouse..
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cystin-1
NCBI Official Synonym Full Names
cystin 1
NCBI Official Symbol
CYS1
NCBI Protein Information
cystin-1
UniProt Protein Name
Cystin-1
Protein Family
UniProt Gene Name
CYS1
Uniprot Description
Enzyme and pathway databasesReactome - a knowledgebase of biological pathways and processesMore...ReactomeiR-HSA-5624138 Trafficking of myristoylated proteins to the cilium
Research Articles on CYS1
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Product Notes
The Human CYS1 cys1 (Catalog #AAA9716073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716073 ELISA Kit recognizes Human CYS1. It is sometimes possible for the material contained within the vial of "Cystin-1 (CYS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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