Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) Standard Curve.)
Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit | PIF/DCD elisa kit
Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) Standard Curve.)
Background: Dermcidin is a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients.High performance liquid chromatography, mass spectrometry, and microsequence analysis of sweat showed a processed 47-amino acid DCD peptide with a calculated mass of 4.7 kD, compared with the expected 9.3 kD of full-length DCD without the signal peptide.DCD expression in skin, melanocytic nevus, and cutaneous melanoma tissue, but not in any other organ systems tested.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Uniprot Description
DCD: DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; EC 3.4.-.-
Chromosomal Location of Human Ortholog: 12q13.1
Cellular Component: extracellular region
Molecular Function: peptidase activity
Biological Process: killing of cells of another organism; defense response to bacterium; proteolysis; defense response to fungus
Research Articles on PIF/DCD
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Product Notes
The Human PIF/DCD dcd (Catalog #AAA9716072) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716072 ELISA Kit recognizes Human PIF/DCD. It is sometimes possible for the material contained within the vial of "Proteolysis Inducing Factor/Dermcidin (PIF/DCD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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