Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Endothelial cell-specific molecule 1 (ESM-1) Standard Curve.)
Human Endothelial Cell-Specific Molecule 1 (ESM-1) ELISA Kit | ESM-1 elisa kit
Human Endothelial Cell-Specific Molecule 1 (ESM-1) ELISA Kit
Gene Names
ESM1; endocan
Reactivity
Human
Synonyms
Endothelial Cell-Specific Molecule 1 (ESM-1); Human Endothelial Cell-Specific Molecule 1 (ESM-1) ELISA Kit; ESM1; endocan; ESM-1 elisa kit
Reactivity
Human
Specificity
Human Endothelial cell-specific molecule 1 (ESM-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ESM1. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
Sequence Length
134
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Endothelial cell-specific molecule 1 (ESM-1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Endothelial cell-specific molecule 1 (ESM-1) Standard Curve.)
Related Product Information for ESM-1 elisa kit
Description: This Human Endothelial cell-specific molecule 1 (ESM-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ESM1 in samples. An antibody specific for ESM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ESM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Endothelial cell-specific molecule 1 is encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. The cDNA sequence contains an open reading frame of 552 nucleotides. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue is restricted to the lung. Immunoblotting and immunoprecipitation identified a 20-kD protein.
Background: Endothelial cell-specific molecule 1 is encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. The cDNA sequence contains an open reading frame of 552 nucleotides. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue is restricted to the lung. Immunoblotting and immunoprecipitation identified a 20-kD protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
endothelial cell-specific molecule 1 isoform b
NCBI Official Synonym Full Names
endothelial cell specific molecule 1
NCBI Official Symbol
ESM1
NCBI Official Synonym Symbols
endocan
NCBI Protein Information
endothelial cell-specific molecule 1
UniProt Protein Name
Endothelial cell-specific molecule 1
Protein Family
UniProt Gene Name
ESM1
UniProt Synonym Gene Names
ESM-1
UniProt Entry Name
ESM1_HUMAN
NCBI Description
This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
ESM1: May have potent implications in lung endothelial cell- leukocyte interactions. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: extracellular region
Molecular Function: integrin binding; insulin-like growth factor binding; hepatocyte growth factor receptor binding
Biological Process: positive regulation of cell proliferation; regulation of cell growth; angiogenesis; sprouting angiogenesis
Research Articles on ESM-1
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Product Notes
The Human ESM-1 esm1 (Catalog #AAA9716059) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716059 ELISA Kit recognizes Human ESM-1. It is sometimes possible for the material contained within the vial of "Endothelial Cell-Specific Molecule 1 (ESM-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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