Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mixed lineage kinase domain-like (MLKL) Standard Curve.)
Human Mixed Lineage Kinase Domain-Like (MLKL) ELISA Kit | MLKL elisa kit
Human Mixed Lineage Kinase Domain-Like (MLKL) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mixed lineage kinase domain-like (MLKL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mixed lineage kinase domain-like (MLKL) Standard Curve.)
Background: MLKL, Belongs to the protein kinase superfamily. The protein kinase domain is predicted to be catalytically inactive. Protein kinases are critical modulators in the signal pathway of tumor cells and therefore a suitable object of drug development using inhibitors for such kinases. Sequence analysis of MLKL identified a kinase domain within the C-terminus of the protein. Further data support the idea that MLKL specifically can regulate more than one signaling pathway. However, the specific function of MLKL was not known yet. The apoptotic activity of MLKL was in fact higher compared to known tumor suppressors such as CDKN1A, PTEN, TP53, or MYC. These findings suggest MLKL as a new target for cancer therapy.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
Uniprot Description
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; TKL-Unique family
Chromosomal Location of Human Ortholog: 16q23.1
Cellular Component: cytoplasm; plasma membrane
Molecular Function: protein binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity
Biological Process: protein amino acid phosphorylation
Research Articles on MLKL
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Product Notes
The Human MLKL mlkl (Catalog #AAA9716029) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716029 ELISA Kit recognizes Human MLKL. It is sometimes possible for the material contained within the vial of "Mixed Lineage Kinase Domain-Like (MLKL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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