Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine protease 27 (PRSS27) Standard Curve.)
Human Serine Protease 27 (PRSS27) ELISA Kit | PRSS27 elisa kit
Human Serine Protease 27 (PRSS27) ELISA Kit
Gene Names
PRSS27; MPN; CAPH2
Reactivity
Human
Synonyms
Serine Protease 27 (PRSS27); Human Serine Protease 27 (PRSS27) ELISA Kit; PRSS27; CAPH2; MPN; channel-activating protease 2; marapsin; pancreasin; PRSS27 elisa kit
Reactivity
Human
Specificity
Human Serine protease 27 (PRSS27) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS27. No significant cross-reactivity or interference between Human PRSS27 and analogues was observed.
Sequence Length
188
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine protease 27 (PRSS27) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine protease 27 (PRSS27) Standard Curve.)
Related Product Information for PRSS27 elisa kit
Description: This Human Serine protease 27 (PRSS27) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS27 in samples. An antibody specific for PRSS27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pancreasin is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. The deduced 290-amino acid preproprotease contains an N-terminal signal peptide, followed by a prosequence and a 256-amino acid serine protease catalytic domain. The catalytic domain contains the essential catalytic triad (his75, asp124, and ser229) conserved in serine proteases, as well as 2 putative N-glycosylation sites. Northern blot analysis revealed expression of pancreasin only in pancreas. RT-PCR detected expression in several other tissues, including strong expression in lung and placenta. Immunocytochemical analysis of pancreatic carcinoma cells revealed cytoplasmic reactivity, and transfected Chinese hamster ovary cells secreted pancreasin into the medium.
Background: Pancreasin is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. The deduced 290-amino acid preproprotease contains an N-terminal signal peptide, followed by a prosequence and a 256-amino acid serine protease catalytic domain. The catalytic domain contains the essential catalytic triad (his75, asp124, and ser229) conserved in serine proteases, as well as 2 putative N-glycosylation sites. Northern blot analysis revealed expression of pancreasin only in pancreas. RT-PCR detected expression in several other tissues, including strong expression in lung and placenta. Immunocytochemical analysis of pancreatic carcinoma cells revealed cytoplasmic reactivity, and transfected Chinese hamster ovary cells secreted pancreasin into the medium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine protease 27 isoform 2
NCBI Official Synonym Full Names
serine protease 27
NCBI Official Symbol
PRSS27
NCBI Official Synonym Symbols
MPN; CAPH2
NCBI Protein Information
serine protease 27
UniProt Protein Name
Serine protease 27
Protein Family
UniProt Gene Name
PRSS27
UniProt Synonym Gene Names
MPN
UniProt Entry Name
PRS27_HUMAN
NCBI Description
This gene is located within a large protease gene cluster on chromosome 16. It belongs to the group-1 subfamily of serine proteases. The encoded protein is a secreted tryptic serine protease and is expressed mainly in the pancreas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
PRSS27: Belongs to the peptidase S1 family
Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Protease
Chromosomal Location of Human Ortholog: 16p13.3
Research Articles on PRSS27
Similar Products
Product Notes
The Human PRSS27 prss27 (Catalog #AAA9716001) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716001 ELISA Kit recognizes Human PRSS27. It is sometimes possible for the material contained within the vial of "Serine Protease 27 (PRSS27), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
