Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Carbonyl reductase [NADPH] 3 (CBR3) Standard Curve.)
Human Carbonyl Reductase [NADPH] 3 (CBR3) ELISA Kit | CBR3 elisa kit
Human Carbonyl Reductase [NADPH] 3 (CBR3) ELISA Kit
Gene Names
CBR3; hCBR3; SDR21C2; HEL-S-25
Reactivity
Human
Synonyms
Carbonyl Reductase [NADPH] 3 (CBR3); Human Carbonyl Reductase [NADPH] 3 (CBR3) ELISA Kit; CBR3; SDR21C2; hCBR3; NADPH-dependent carbonyl reductase 3; carbonyl reductase (NADPH) 3; short chain dehydrogenase/reductase family 21C; member 2; CBR3 elisa kit
Reactivity
Human
Specificity
Human Carbonyl reductase [NADPH] 3 (CBR3) ELISA Kit has high sensitivity and excellent specificity for detection of Human CBR3. No significant cross-reactivity or interference between Human CBR3 and analogues was observed.
Sequence Length
277
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Carbonyl reductase [NADPH] 3 (CBR3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Carbonyl reductase [NADPH] 3 (CBR3) Standard Curve.)
Related Product Information for CBR3 elisa kit
Description: This Human Carbonyl reductase [NADPH] 3 (CBR3) ELISA Kit employs a two-site sandwich ELISA to quantitate CBR3 in samples. An antibody specific for CBR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. Carbonyl reductase (CBR) catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. By analyzing sequences from the 21q22.2 chromosomal region, Watanabe et al. (1998) identified genomic clones corresponding to CBR1 and to CBR3, a novel carbonyl reductase gene. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein.
Background: Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. Carbonyl reductase (CBR) catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. By analyzing sequences from the 21q22.2 chromosomal region, Watanabe et al. (1998) identified genomic clones corresponding to CBR1 and to CBR3, a novel carbonyl reductase gene. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
carbonyl reductase
NCBI Official Synonym Full Names
carbonyl reductase 3
NCBI Official Symbol
CBR3
NCBI Official Synonym Symbols
hCBR3; SDR21C2; HEL-S-25
NCBI Protein Information
carbonyl reductase [NADPH] 3
UniProt Protein Name
Carbonyl reductase [NADPH] 3
Protein Family
UniProt Gene Name
CBR3
UniProt Entry Name
CBR3_HUMAN
NCBI Description
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008]
Uniprot Description
CBR3: Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Oxidoreductase; EC 1.1.1.184; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 21q22.2
Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol
Molecular Function: 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity
Biological Process: phylloquinone catabolic process; cognition
Research Articles on CBR3
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Product Notes
The Human CBR3 cbr3 (Catalog #AAA9715953) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715953 ELISA Kit recognizes Human CBR3. It is sometimes possible for the material contained within the vial of "Carbonyl Reductase [NADPH] 3 (CBR3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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