Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydropyrimidinase (DPYS) Standard Curve.)
Human Dihydropyrimidinase (DPYS) ELISA Kit | DPYS elisa kit
Human Dihydropyrimidinase (DPYS) ELISA Kit
Gene Names
DPYS; DHP; DHPase
Reactivity
Human
Synonyms
Dihydropyrimidinase (DPYS); Human Dihydropyrimidinase (DPYS) ELISA Kit; DPYS; DHP; DHPase; dihydropyrimidine amidohydrolase; hydantoinase; DPYS elisa kit
Reactivity
Human
Specificity
Human Dihydropyrimidinase (DPYS) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPYS. No significant cross-reactivity or interference between Human DPYS and analogues was observed.
Sequence Length
519
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydropyrimidinase (DPYS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydropyrimidinase (DPYS) Standard Curve.)
Related Product Information for DPYS elisa kit
Description: This Human Dihydropyrimidinase (DPYS) ELISA Kit employs a two-site sandwich ELISA to quantitate DPYS in samples. An antibody specific for DPYS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPYS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPYS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPYS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.Various neurologic abnormalities have been described in this group of patients. The further catabolism of the dihydropyrimidines is effected by dihydropyrimidinase. Duran et al. reported a male Turkish baby, the fifth child of a consanguineous couple, who was thought to have dihydropyrimidinase deficiency. The infant was well until the age of 8 weeks when he developed feeding problems following the introduction of fruits to his formula milk.
Background: Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.Various neurologic abnormalities have been described in this group of patients. The further catabolism of the dihydropyrimidines is effected by dihydropyrimidinase. Duran et al. reported a male Turkish baby, the fifth child of a consanguineous couple, who was thought to have dihydropyrimidinase deficiency. The infant was well until the age of 8 weeks when he developed feeding problems following the introduction of fruits to his formula milk.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dihydropyrimidinase
NCBI Official Synonym Full Names
dihydropyrimidinase
NCBI Official Symbol
DPYS
NCBI Official Synonym Symbols
DHP; DHPase
NCBI Protein Information
dihydropyrimidinase
UniProt Protein Name
Dihydropyrimidinase
UniProt Gene Name
DPYS
UniProt Synonym Gene Names
DHP; DHPase
NCBI Description
Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
Research Articles on DPYS
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Product Notes
The Human DPYS dpys (Catalog #AAA9715644) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715644 ELISA Kit recognizes Human DPYS. It is sometimes possible for the material contained within the vial of "Dihydropyrimidinase (DPYS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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