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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Malectin (MLEC) Standard Curve.)

Human Malectin (MLEC) ELISA Kit | MLEC elisa kit

Human Malectin (MLEC) ELISA Kit

Gene Names
MLEC; KIAA0152
Reactivity
Human
Synonyms
Malectin (MLEC); Human Malectin (MLEC) ELISA Kit; MLEC; KIAA0152; MLEC elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Malectin (MLEC) ELISA Kit has high sensitivity and excellent specificity for detection of Human MLEC. No significant cross-reactivity or interference between Human MLEC and analogues was observed.
Sequence Length
292
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Malectin (MLEC) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Malectin (MLEC) Standard Curve.)
Related Product Information for MLEC elisa kit
Description: This Human Malectin (MLEC) ELISA Kit employs a two-site sandwich ELISA to quantitate MLEC in samples. An antibody specific for MLEC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLEC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLEC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLEC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MLEC encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. Malectin has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. Malectin has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
malectin isoform1
NCBI Official Synonym Full Names
malectin
NCBI Official Symbol
MLEC
NCBI Official Synonym Symbols
KIAA0152
NCBI Protein Information
malectin
UniProt Protein Name
Malectin
Protein Family
UniProt Gene Name
MLEC
UniProt Synonym Gene Names
KIAA0152
UniProt Entry Name
MLEC_HUMAN

NCBI Description

This gene encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

MLEC: Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Belongs to the malectin family.

Protein type: Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: enzyme binding; carbohydrate binding

Biological Process: cellular protein metabolic process; protein folding; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Research Articles on MLEC

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Product Notes

The Human MLEC mlec (Catalog #AAA9715265) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715265 ELISA Kit recognizes Human MLEC. It is sometimes possible for the material contained within the vial of "Malectin (MLEC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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