Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymus-specific serine protease (PRSS16) Standard Curve.)
Human Thymus-Specific Serine Protease (PRSS16) ELISA Kit | TSSP elisa kit
Human Thymus-Specific Serine Protease (PRSS16) ELISA Kit
Gene Names
PRSS16; TSSP
Reactivity
Human
Synonyms
Thymus-Specific Serine Protease (PRSS16); Human Thymus-Specific Serine Protease (PRSS16) ELISA Kit; PRSS16; XXbac-BPG24O18.5; FLJ36271; FLJ40714; FLJ44172; protease; serine; 16; thymus specific serine peptidase; TSSP elisa kit
Reactivity
Human
Specificity
Human Thymus-specific serine protease (PRSS16) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS16. No significant cross-reactivity or interference between Human PRSS16 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymus-specific serine protease (PRSS16) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymus-specific serine protease (PRSS16) Standard Curve.)
Related Product Information for TSSP elisa kit
Description: This Human Thymus-specific serine protease (PRSS16) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS16 in samples. An antibody specific for PRSS16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TSSP is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6, near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described, but its full length nature has not been determined.The deduced 514-amino acid PRSS16 protein, which the authors termed TSSP, has a calculated molecular mass of 55 kD. PRSS16 contains a putative N-terminal signal peptide and a prolylendopeptidase consensus sequence. Within its 3-prime untranslated region, it has 2 different polyadenylation sites. PRSS16 shares sequence similarity with prolylcarboxypeptidase (PRCP), particularly around the active-site serine.
Background: TSSP is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6, near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described, but its full length nature has not been determined.The deduced 514-amino acid PRSS16 protein, which the authors termed TSSP, has a calculated molecular mass of 55 kD. PRSS16 contains a putative N-terminal signal peptide and a prolylendopeptidase consensus sequence. Within its 3-prime untranslated region, it has 2 different polyadenylation sites. PRSS16 shares sequence similarity with prolylcarboxypeptidase (PRCP), particularly around the active-site serine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Synonym Full Names
serine protease 16
NCBI Official Symbol
PRSS16
NCBI Official Synonym Symbols
TSSP
NCBI Protein Information
thymus-specific serine protease
UniProt Protein Name
Thymus-specific serine protease
UniProt Gene Name
PRSS16
UniProt Synonym Gene Names
TSSP
UniProt Entry Name
TSSP_HUMAN
NCBI Description
This gene encodes a serine protease expressed exclusively in the thymus. It is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6, near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Protease that may play a role in T-cell development.
Subcellular location: Cytoplasmic vesicle. Note: Vesicular, either lysosomal or endosomal.
Tissue specificity: Expressed predominantly in cortical thymic epithelial cells.
Developmental stage: Expressed in fetal thymus.
Sequence similarities: Belongs to the peptidase S28 family.
Sequence caution: The sequence CAB94769.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on TSSP
Similar Products
Product Notes
The Human TSSP prss16 (Catalog #AAA9714776) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714776 ELISA Kit recognizes Human TSSP. It is sometimes possible for the material contained within the vial of "Thymus-Specific Serine Protease (PRSS16), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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