Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Brain-specific serine protease 4 (PRSS22) Standard Curve.)
Human Brain-Specific Serine Protease 4 (PRSS22) ELISA Kit | PRSS22 elisa kit
Human Brain-Specific Serine Protease 4 (PRSS22) ELISA Kit
Gene Names
PRSS22; BSSP-4; SP001LA; hBSSP-4
Reactivity
Human
Synonyms
Brain-Specific Serine Protease 4 (PRSS22); Human Brain-Specific Serine Protease 4 (PRSS22) ELISA Kit; PRSS22; BSSP-4; MGC9599; SP001LA; hBSSP-4; brain-specific serine protease 4; prosemin; protease; serine S1 family member 22; tryptase epsilon; PRSS22 elisa kit
Reactivity
Human
Specificity
Human Brain-specific serine protease 4 (PRSS22) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS22. No significant cross-reactivity or interference between Human PRSS22 and analogues was observed.
Sequence Length
317
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Brain-specific serine protease 4 (PRSS22) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Brain-specific serine protease 4 (PRSS22) Standard Curve.)
Related Product Information for PRSS22 elisa kit
Description: This Human Brain-specific serine protease 4 (PRSS22) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS22 in samples. An antibody specific for PRSS22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 317-amino acid protein contains an N-terminal hydrophobic leader peptide, followed by a propeptide sequence and the serine protease domain, which has the catalytic triad of histidine, aspartic acid, and serine. PRSS22 also has 2 cysteines that form a disulfide bond linking the propeptide and the catalytic domain following proteolytic activation, and it has an N-glycosylation site. PRSS22 lacks a number of residues required by other tryptases to form tetramers. RNA dot blot analysis detected abundant PRSS22 expression in adult esophagus and trachea, with lower levels in adult placenta, pancreas, prostate, and thyroid, and in fetal kidney and lung. There was little to no expression in other tissues examined. Enzymatically active PRSS22 was secreted from a human epithelial cell line and from transfected COS-7 cells.
Background: The deduced 317-amino acid protein contains an N-terminal hydrophobic leader peptide, followed by a propeptide sequence and the serine protease domain, which has the catalytic triad of histidine, aspartic acid, and serine. PRSS22 also has 2 cysteines that form a disulfide bond linking the propeptide and the catalytic domain following proteolytic activation, and it has an N-glycosylation site. PRSS22 lacks a number of residues required by other tryptases to form tetramers. RNA dot blot analysis detected abundant PRSS22 expression in adult esophagus and trachea, with lower levels in adult placenta, pancreas, prostate, and thyroid, and in fetal kidney and lung. There was little to no expression in other tissues examined. Enzymatically active PRSS22 was secreted from a human epithelial cell line and from transfected COS-7 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
brain-specific serine protease 4
NCBI Official Synonym Full Names
serine protease 22
NCBI Official Symbol
PRSS22
NCBI Official Synonym Symbols
BSSP-4; SP001LA; hBSSP-4
NCBI Protein Information
brain-specific serine protease 4
UniProt Protein Name
Brain-specific serine protease 4
Protein Family
UniProt Gene Name
PRSS22
UniProt Synonym Gene Names
BSSP4; PRSS26; SP001LA; UNQ302/PRO343; BSSP-4
UniProt Entry Name
BSSP4_HUMAN
NCBI Description
This gene encodes a member of the trypsin family of serine proteases. The enzyme is expressed in the airways in a developmentally regulated manner. The gene is part of a cluster of serine protease genes on chromosome 16. [provided by RefSeq, Jul 2008]
Uniprot Description
PRSS22: Preferentially cleaves the synthetic substrate H-D-Leu- Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA. Belongs to the peptidase S1 family.
Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.-
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on PRSS22
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Product Notes
The Human PRSS22 prss22 (Catalog #AAA9714773) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714773 ELISA Kit recognizes Human PRSS22. It is sometimes possible for the material contained within the vial of "Brain-Specific Serine Protease 4 (PRSS22), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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