Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine protease 57 (PRSSL1) Standard Curve.)
Human Serine Protease 57 (PRSSL1) ELISA Kit | PRSSL1 elisa kit
Human Serine Protease 57 (PRSSL1) ELISA Kit
Gene Names
PRSS57; NSP4; PRSSL1; UNQ782
Reactivity
Human
Synonyms
Serine Protease 57 (PRSSL1); Human Serine Protease 57 (PRSSL1) ELISA Kit; PRSSL1; UNQ782; GLGL782; PRSSL1 elisa kit
Reactivity
Human
Specificity
Human Serine protease 57 (PRSSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSSL1. No significant cross-reactivity or interference between Human PRSSL1 and analogues was observed.
Sequence Length
283
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine protease 57 (PRSSL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine protease 57 (PRSSL1) Standard Curve.)
Related Product Information for PRSSL1 elisa kit
Description: This Human Serine protease 57 (PRSSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSSL1 in samples. An antibody specific for PRSSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRSSL1 encodes an arginine-specific serine protease and member of the peptidase S1 family of proteins. The encoded protein may undergo proteolytic activation before storage in azurophil granules, in neutrophil cells of the immune system. Following neutrophil activation, the protease is released into the pericellular environment, where it may play a role in defense against microbial pathogens.
Background: PRSSL1 encodes an arginine-specific serine protease and member of the peptidase S1 family of proteins. The encoded protein may undergo proteolytic activation before storage in azurophil granules, in neutrophil cells of the immune system. Following neutrophil activation, the protease is released into the pericellular environment, where it may play a role in defense against microbial pathogens.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine protease 57 isoform 1
NCBI Official Synonym Full Names
serine protease 57
NCBI Official Symbol
PRSS57
NCBI Official Synonym Symbols
NSP4; PRSSL1; UNQ782
NCBI Protein Information
serine protease 57
UniProt Protein Name
Serine protease 57
UniProt Gene Name
PRSS57
UniProt Synonym Gene Names
PRSSL1; UNQ782/PRO1599
UniProt Entry Name
PRS57_HUMAN
NCBI Description
This gene encodes an arginine-specific serine protease and member of the peptidase S1 family of proteins. The encoded protein may undergo proteolytic activation before storage in azurophil granules, in neutrophil cells of the immune system. Following neutrophil activation, the protease is released into the pericellular environment, where it may play a role in defense against microbial pathogens. [provided by RefSeq, Jul 2016]
Uniprot Description
PRSSL1: Belongs to the peptidase S1 family.
Protein type: EC 3.4.21.-; Secreted, signal peptide; Mitochondrial; Protease; Secreted
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on PRSSL1
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Product Notes
The Human PRSSL1 prss57 (Catalog #AAA9714764) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714764 ELISA Kit recognizes Human PRSSL1. It is sometimes possible for the material contained within the vial of "Serine Protease 57 (PRSSL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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