Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) Standard Curve.)
Human Receptor-Type Tyrosine-Protein Phosphatase N2 (PTPRN2) ELISA Kit | PTPRN2 elisa kit
Human Receptor-Type Tyrosine-Protein Phosphatase N2 (PTPRN2) ELISA Kit
Gene Names
PTPRN2; IAR; ICAAR; PTPRP; IA-2beta; R-PTP-N2
Reactivity
Human
Synonyms
Receptor-Type Tyrosine-Protein Phosphatase N2 (PTPRN2); Human Receptor-Type Tyrosine-Protein Phosphatase N2 (PTPRN2) ELISA Kit; PTPRN2; IA-2beta; IAR; ICAAR; PTPRP; IAR/receptor-like protein-tyrosine phosphatase; phogrin; protein tyrosine phosphatase receptor pi; tyrosine phosphatase IA-2 beta; PTPRN2 elisa kit
Reactivity
Human
Specificity
Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPRN2. No significant cross-reactivity or interference between Human PTPRN2 and analogues was observed.
Sequence Length
1015
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) Standard Curve.)
Related Product Information for PTPRN2 elisa kit
Description: This Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRN2 in samples. An antibody specific for PTPRN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTPRN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Receptor-type tyrosine-protein phosphatase N2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. The catalytic domain of this PTP is most closely related to PTPRN/IA-2beta. This PTP and PTPRN are both found to be major autoantigens associated with insulin-dependent diabetes mellitus. Three alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Background: Receptor-type tyrosine-protein phosphatase N2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. The catalytic domain of this PTP is most closely related to PTPRN/IA-2beta. This PTP and PTPRN are both found to be major autoantigens associated with insulin-dependent diabetes mellitus. Three alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase N2 isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase receptor type N2
NCBI Official Symbol
PTPRN2
NCBI Official Synonym Symbols
IAR; ICAAR; PTPRP; IA-2beta; R-PTP-N2
NCBI Protein Information
receptor-type tyrosine-protein phosphatase N2
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase N2
Protein Family
UniProt Gene Name
PTPRN2
UniProt Synonym Gene Names
KIAA0387; R-PTP-N2; IAR; ICAAR
UniProt Entry Name
PTPR2_HUMAN
NCBI Description
This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Research Articles on PTPRN2
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Product Notes
The Human PTPRN2 ptprn2 (Catalog #AAA9714711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714711 ELISA Kit recognizes Human PTPRN2. It is sometimes possible for the material contained within the vial of "Receptor-Type Tyrosine-Protein Phosphatase N2 (PTPRN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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