Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) Standard Curve.)
Human Acyl-CoA Synthetase Family Member 2, Mitochondrial (ACSF2) ELISA Kit | ACSF2 elisa kit
Human Acyl-CoA Synthetase Family Member 2, Mitochondrial (ACSF2) ELISA Kit
Gene Names
ACSF2; ACSMW; AVYV493
Reactivity
Human
Synonyms
Acyl-CoA Synthetase Family Member 2; Mitochondrial (ACSF2); Human Acyl-CoA Synthetase Family Member 2; Mitochondrial (ACSF2) ELISA Kit; ACSF2; ACSMW; AVYV493; FLJ20920; PPARG binding; long chain fatty acid acyl Co-A ligase like; ACSF2 elisa kit
Reactivity
Human
Specificity
Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACSF2. No significant cross-reactivity or interference between Human ACSF2 and analogues was observed.
Sequence Length
640
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) Standard Curve.)
Related Product Information for ACSF2 elisa kit
Description: This Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSF2 in samples. An antibody specific for ACSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Fatty acids are incorporated into membranes and signaling molecules and have roles in energy storage and metabolism. These essential functions require activation of the fatty acid by acyl-coenzyme A (CoA) synthetases, such as ACSF2, which form an activating thioester linkage between the fatty acid and CoA.The deduced 615-amino acid protein contains all 5 motifs characteristic of acyl-CoA synthetases. Phylogenetic analysis revealed that ACSF2 belongs to a small group of acyl-CoA synthetases that differ significantly from all other clades of acyl-CoA synthetases and from each other. FLJ20920 contains a PPARG-recognition element in its promoter sequence. Transfection of antisense FLJ20920 into preadipocytes resulted in decreased triglyceride accumulation and reduced expression of GLUT4 (SLC2A4) and HSL (LIPE) mRNA.
Background: Fatty acids are incorporated into membranes and signaling molecules and have roles in energy storage and metabolism. These essential functions require activation of the fatty acid by acyl-coenzyme A (CoA) synthetases, such as ACSF2, which form an activating thioester linkage between the fatty acid and CoA.The deduced 615-amino acid protein contains all 5 motifs characteristic of acyl-CoA synthetases. Phylogenetic analysis revealed that ACSF2 belongs to a small group of acyl-CoA synthetases that differ significantly from all other clades of acyl-CoA synthetases and from each other. FLJ20920 contains a PPARG-recognition element in its promoter sequence. Transfection of antisense FLJ20920 into preadipocytes resulted in decreased triglyceride accumulation and reduced expression of GLUT4 (SLC2A4) and HSL (LIPE) mRNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
acyl-CoA synthetase family member 2, mitochondrial isoform 1
NCBI Official Synonym Full Names
acyl-CoA synthetase family member 2
NCBI Official Symbol
ACSF2
NCBI Official Synonym Symbols
ACSMW; AVYV493
NCBI Protein Information
acyl-CoA synthetase family member 2, mitochondrial
UniProt Protein Name
Acyl-CoA synthetase family member 2, mitochondrial
Protein Family
UniProt Gene Name
ACSF2
UniProt Entry Name
ACSF2_HUMAN
Uniprot Description
ACSF2: Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipodyte differentiation. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: EC 6.2.1.-; Ligase
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: mitochondrial matrix
Molecular Function: acyl-CoA ligase activity; protein binding
Research Articles on ACSF2
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Product Notes
The Human ACSF2 acsf2 (Catalog #AAA9714551) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714551 ELISA Kit recognizes Human ACSF2. It is sometimes possible for the material contained within the vial of "Acyl-CoA Synthetase Family Member 2, Mitochondrial (ACSF2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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