Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S100-A16 (S100A16) Standard Curve.)
Human Protein S100-A16 (S100A16) ELISA Kit | S100A16 elisa kit
Human Protein S100-A16 (S100A16) ELISA Kit
Gene Names
S100A16; AAG13; S100F; DT1P1A7
Reactivity
Human
Synonyms
Protein S100-A16 (S100A16); Human Protein S100-A16 (S100A16) ELISA Kit; S100A16; AAG13; DT1P1A7; MGC17528; S100F; OTTHUMP00000015465; OTTHUMP00000015466; OTTHUMP00000032894; aging-associated protein 13; S100A16 elisa kit
Reactivity
Human
Specificity
Human Protein S100-A16 (S100A16) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A16. No significant cross-reactivity or interference between Human S100A16 and analogues was observed.
Sequence Length
103
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S100-A16 (S100A16) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S100-A16 (S100A16) Standard Curve.)
Related Product Information for S100A16 elisa kit
Description: This Human Protein S100-A16 (S100A16) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A16 in samples. An antibody specific for S100A16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protein S100-A16 is a protein encoded by the S100A16 gene.S-100 protein is a family of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH.Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.
Background: Protein S100-A16 is a protein encoded by the S100A16 gene.S-100 protein is a family of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH.Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein S100-A16
NCBI Official Synonym Full Names
S100 calcium binding protein A16
NCBI Official Symbol
S100A16
NCBI Official Synonym Symbols
AAG13; S100F; DT1P1A7
NCBI Protein Information
protein S100-A16
UniProt Protein Name
Protein S100-A16
Protein Family
UniProt Gene Name
S100A16
UniProt Synonym Gene Names
S100F
UniProt Entry Name
S10AG_HUMAN
Uniprot Description
S100A16: Calcium-binding protein. Binds one calcium ion per monomer. Belongs to the S-100 family.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; nucleolus; plasma membrane; cytosol
Molecular Function: protein homodimerization activity; calcium ion binding
Biological Process: response to calcium ion
Research Articles on S100A16
Similar Products
Product Notes
The Human S100A16 s100a16 (Catalog #AAA9714432) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714432 ELISA Kit recognizes Human S100A16. It is sometimes possible for the material contained within the vial of "Protein S100-A16 (S100A16), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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