Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-3B (SEMA3B) Standard Curve.)
Human Semaphorin-3B (SEMA3B) ELISA Kit | SEMA3B elisa kit
Human Semaphorin-3B (SEMA3B) ELISA Kit
Gene Names
SEMA3B; SemA; SEMA5; SEMAA; semaV; LUCA-1
Reactivity
Human
Synonyms
Semaphorin-3B (SEMA3B); Human Semaphorin-3B (SEMA3B) ELISA Kit; SEMA3B; FLJ34863; LUCA-1; SEMA5; SEMAA; SemA; semaV; semaphorin 3B; semaphorin A; semaphorin V; SEMA3B elisa kit
Reactivity
Human
Specificity
Human Semaphorin-3B (SEMA3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA3B. No significant cross-reactivity or interference between Human SEMA3B and analogues was observed.
Sequence Length
749
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-3B (SEMA3B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-3B (SEMA3B) Standard Curve.)
Related Product Information for SEMA3B elisa kit
Description: This Human Semaphorin-3B (SEMA3B) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3B in samples. An antibody specific for SEMA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis. The 2 genes have widespread but distinct patterns of expression in nonneural tissues, and have different patterns of expression in lung cancer. Human semaphorin A(V) has 86% amino acid homology with murine semaphorin A, whereas semaphorin IV is more closely related to murine semaphorin E, with 50% homology.In particular, overlapping homozygous deletions in lung cancers have been identified in region 3p21.3; more than 90% of small cell lung cancers exhibit LOH at this site.
Background: The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis. The 2 genes have widespread but distinct patterns of expression in nonneural tissues, and have different patterns of expression in lung cancer. Human semaphorin A(V) has 86% amino acid homology with murine semaphorin A, whereas semaphorin IV is more closely related to murine semaphorin E, with 50% homology.In particular, overlapping homozygous deletions in lung cancers have been identified in region 3p21.3; more than 90% of small cell lung cancers exhibit LOH at this site.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
semaphorin-3B isoform 1
NCBI Official Synonym Full Names
semaphorin 3B
NCBI Official Symbol
SEMA3B
NCBI Official Synonym Symbols
SemA; SEMA5; SEMAA; semaV; LUCA-1
NCBI Protein Information
semaphorin-3B
UniProt Protein Name
Semaphorin-3B
Protein Family
UniProt Gene Name
SEMA3B
UniProt Synonym Gene Names
SEMA5; SEMAA; Sema V
NCBI Description
The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Research Articles on SEMA3B
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Product Notes
The Human SEMA3B sema3b (Catalog #AAA9714381) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714381 ELISA Kit recognizes Human SEMA3B. It is sometimes possible for the material contained within the vial of "Semaphorin-3B (SEMA3B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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