Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Macoilin (TMEM57) Standard Curve.)
Human Macoilin (TMEM57) ELISA Kit | TMEM57 elisa kit
Human Macoilin (TMEM57) ELISA Kit
Gene Names
MACO1; TMEM57; MACOILIN
Reactivity
Human
Synonyms
Macoilin (TMEM57); Human Macoilin (TMEM57) ELISA Kit; TMEM57; RP3-469D22.2; FLJ10747; FLJ23007; MACOILIN; TMEM57 elisa kit
Reactivity
Human
Specificity
Human Macoilin (TMEM57) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM57. No significant cross-reactivity or interference between Human TMEM57 and analogues was observed.
Sequence Length
664
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Macoilin (TMEM57) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Macoilin (TMEM57) Standard Curve.)
Related Product Information for TMEM57 elisa kit
Description: This Human Macoilin (TMEM57) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM57 in samples. An antibody specific for TMEM57 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM57 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM57 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM57 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMEM57 has 5 domains, including an N-terminal LAG1 domain and a central ERM-myosin filament domain. The LAG1 domain contains 3 putative transmembrane domains. In situ hybridization of mouse embryos showed that Tmem57 was brain specific. It was primarily associated with postmigratory neurons and less so with neural proliferating cells, and Tmem57 expression was progressively downregulated during brain maturation. Fluorescence-tagged Tmem57 outlined the nuclear membrane when expressed in nonneuronal cells. In primary hippocampal neurons, Tmem57 was found in neurites and their terminals, and it partially colocalized with synapsin-1 (SYN1), a presynaptic marker.
Background: TMEM57 has 5 domains, including an N-terminal LAG1 domain and a central ERM-myosin filament domain. The LAG1 domain contains 3 putative transmembrane domains. In situ hybridization of mouse embryos showed that Tmem57 was brain specific. It was primarily associated with postmigratory neurons and less so with neural proliferating cells, and Tmem57 expression was progressively downregulated during brain maturation. Fluorescence-tagged Tmem57 outlined the nuclear membrane when expressed in nonneuronal cells. In primary hippocampal neurons, Tmem57 was found in neurites and their terminals, and it partially colocalized with synapsin-1 (SYN1), a presynaptic marker.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Transmembrane protein 57
NCBI Official Synonym Full Names
macoilin 1
NCBI Official Symbol
MACO1
NCBI Official Synonym Symbols
TMEM57; MACOILIN
NCBI Protein Information
macoilin
UniProt Protein Name
Macoilin
Protein Family
UniProt Gene Name
TMEM57
UniProt Entry Name
MACOI_HUMAN
Uniprot Description
Subcellular location: Nucleus membrane; Multi-pass membrane protein
By similarity. Cell projection › axon
By similarity. Note: Detected in the nucleus membrane of non-neuronal cells and in axonal outgrowths of neuronal cells
By similarity.
Sequence similarities: Belongs to the macoilin family.
Research Articles on TMEM57
Similar Products
Product Notes
The Human TMEM57 tmem57 (Catalog #AAA9713942) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713942 ELISA Kit recognizes Human TMEM57. It is sometimes possible for the material contained within the vial of "Macoilin (TMEM57), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
