Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human AN1-type zinc finger protein 6 (ZFAND6) Standard Curve.)
Human AN1-Type Zinc Finger Protein 6 (ZFAND6) ELISA Kit | ZFAND6 elisa kit
Human AN1-Type Zinc Finger Protein 6 (ZFAND6) ELISA Kit
Gene Names
ZFAND6; AWP1; ZA20D3; ZFAND5B
Reactivity
Human
Synonyms
AN1-Type Zinc Finger Protein 6 (ZFAND6); Human AN1-Type Zinc Finger Protein 6 (ZFAND6) ELISA Kit; ZFAND6; AWP1; ZA20D3; ZFAND5B; protein associated with PRK1; zinc finger; A20 domain containing 3; ZFAND6 elisa kit
Reactivity
Human
Specificity
Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZFAND6. No significant cross-reactivity or interference between Human ZFAND6 and analogues was observed.
Sequence Length
208
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human AN1-type zinc finger protein 6 (ZFAND6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human AN1-type zinc finger protein 6 (ZFAND6) Standard Curve.)
Related Product Information for ZFAND6 elisa kit
Description: This Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit employs a two-site sandwich ELISA to quantitate ZFAND6 in samples. An antibody specific for ZFAND6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZFAND6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFAND6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFAND6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 208-amino acid AWP1 protein has a calculated molecular mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216. AWP1 has 2 conserved zinc finger domains: ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic reticulum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.
Background: The deduced 208-amino acid AWP1 protein has a calculated molecular mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216. AWP1 has 2 conserved zinc finger domains: ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic reticulum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
AN1-type zinc finger protein 6 isoform a
NCBI Official Synonym Full Names
zinc finger AN1-type containing 6
NCBI Official Symbol
ZFAND6
NCBI Official Synonym Symbols
AWP1; ZA20D3; ZFAND5B
NCBI Protein Information
AN1-type zinc finger protein 6
UniProt Protein Name
AN1-type zinc finger protein 6
Protein Family
UniProt Gene Name
ZFAND6
UniProt Synonym Gene Names
AWP1; ZA20D3
Uniprot Description
Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes ().
Research Articles on ZFAND6
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Product Notes
The Human ZFAND6 zfand6 (Catalog #AAA9713672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713672 ELISA Kit recognizes Human ZFAND6. It is sometimes possible for the material contained within the vial of "AN1-Type Zinc Finger Protein 6 (ZFAND6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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