Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat N-acetylaspartate synthetase (NAT8L) Standard Curve.)
Rat N-Acetylaspartate Synthetase (NAT8L) ELISA Kit | NAT8L elisa kit
Rat N-Acetylaspartate Synthetase (NAT8L) ELISA Kit
Gene Names
Nat8l; RGD1305719
Reactivity
Rat
Synonyms
N-Acetylaspartate Synthetase (NAT8L); Rat N-Acetylaspartate Synthetase (NAT8L) ELISA Kit; NAT8L; CML3; FLJ37478; MGC117272; NAT8-LIKE; NAT8L elisa kit
Reactivity
Rat
Specificity
Rat N-acetylaspartate synthetase (NAT8L) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NAT8L. No significant cross-reactivity or interference between Rat NAT8L and analogues was observed.
Sequence Length
299
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat N-acetylaspartate synthetase (NAT8L) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat N-acetylaspartate synthetase (NAT8L) Standard Curve.)
Related Product Information for NAT8L elisa kit
Description: This Rat N-acetylaspartate synthetase (NAT8L) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT8L in samples. An antibody specific for NAT8L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAT8L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT8L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT8L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NAT8L encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein NAT8L is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. NAT8L mutation results in primary NAA deficiency (hypoacetylaspartia).
Background: NAT8L encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein NAT8L is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. NAT8L mutation results in primary NAA deficiency (hypoacetylaspartia).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
N-acetylaspartate synthetase
NCBI Official Synonym Full Names
N-acetyltransferase 8-like
NCBI Official Symbol
Nat8l
NCBI Official Synonym Symbols
RGD1305719
NCBI Protein Information
N-acetylaspartate synthetase
UniProt Protein Name
N-acetylaspartate synthetase
Protein Family
UniProt Gene Name
Nat8l
UniProt Synonym Gene Names
NAA synthetase
UniProt Entry Name
NAT8L_RAT
Uniprot Description
Function: Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake
Catalytic activity: Acetyl-CoA + L-aspartate = CoA + N-acetyl-L-aspartate.
Enzyme regulation: Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria
Research Articles on NAT8L
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Product Notes
The Rat NAT8L nat8l (Catalog #AAA9712162) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712162 ELISA Kit recognizes Rat NAT8L. It is sometimes possible for the material contained within the vial of "N-Acetylaspartate Synthetase (NAT8L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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