Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Urokinase plasminogen activator (uPA) Standard Curve.)
Rat Urokinase Plasminogen Activator (uPA) ELISA Kit | uPA elisa kit
Rat Urokinase Plasminogen Activator (uPA) ELISA Kit
Reactivity
Rat
Synonyms
Urokinase Plasminogen Activator (uPA); Rat Urokinase Plasminogen Activator (uPA) ELISA Kit; PLAU; ATF; UPA; URK; u-PA; U-plasminogen activator; plasminogen activator; urinary; urokinase-type plasminogen activator; uPA elisa kit
Reactivity
Rat
Specificity
Rat Urokinase plasminogen activator (uPA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLAU. No significant cross-reactivity or interference between Rat PLAU and analogues was observed.
Sequence Length
433
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Urokinase plasminogen activator (uPA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Urokinase plasminogen activator (uPA) Standard Curve.)
Related Product Information for uPA elisa kit
Description: This Rat Urokinase plasminogen activator (uPA) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAU in samples. An antibody specific for PLAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAU is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAU bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Urokinase-type plasminogen activator is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.
Background: Urokinase-type plasminogen activator is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
urokinase-type plasminogen activator
NCBI Official Symbol
PLAU
NCBI Protein Information
urokinase-type plasminogen activator
UniProt Protein Name
Urokinase-type plasminogen activator
UniProt Gene Name
PLAU
UniProt Synonym Gene Names
U-plasminogen activator; uPA
UniProt Entry Name
UROK_RABIT
Uniprot Description
Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Catalytic activity: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Enzyme regulation: Inhibited by SERPINA5
By similarity.
Subunit structure: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR
By similarity.
Subcellular location: Secreted
By similarity.
Post-translational modification: Phosphorylation of Ser-325 abolishes proadhesive ability but does not interfere with receptor binding
By similarity.
Sequence similarities: Belongs to the peptidase S1 family.Contains 1 EGF-like domain.Contains 1 kringle domain.Contains 1 peptidase S1 domain.
Research Articles on uPA
Similar Products
Product Notes
The Rat uPA plau (Catalog #AAA9712109) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712109 ELISA Kit recognizes Rat uPA. It is sometimes possible for the material contained within the vial of "Urokinase Plasminogen Activator (uPA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
