Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Prostate tumor-overexpressed gene 1 protein (PTOV1) Standard Curve.)
Rat Prostate Tumor-Overexpressed Gene 1 Protein (PTOV1) ELISA Kit | PTOV1 elisa kit
Rat Prostate Tumor-Overexpressed Gene 1 Protein (PTOV1) ELISA Kit
Reactivity
Rat
Synonyms
Prostate Tumor-Overexpressed Gene 1 Protein (PTOV1); Rat Prostate Tumor-Overexpressed Gene 1 Protein (PTOV1) ELISA Kit; PTOV1; ACID2; DKFZp586I111; MGC71475; prostate tumor overexpressed gene 1; PTOV1 elisa kit
Reactivity
Rat
Specificity
Rat Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTOV1. No significant cross-reactivity or interference between Rat PTOV1 and analogues was observed.
Sequence Length
416
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Prostate tumor-overexpressed gene 1 protein (PTOV1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Prostate tumor-overexpressed gene 1 protein (PTOV1) Standard Curve.)
Related Product Information for PTOV1 elisa kit
Description: This Rat Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTOV1 in samples. An antibody specific for PTOV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTOV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTOV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTOV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PTOV1 was predicted to form alternating alpha helices and beta sheets. Normal prostate expressed low PTOV1 levels. Western blot analysis of prostate tumor tissue detected PTOV1 at an apparent molecular mass of 58 kD. Immunocytochemical analysis detected endogenous PTOV1 in the cytoplasm, concentrated around the nucleus.PTOV1 was overexpressed in 71% of 38 prostate carcinomas and in 80% of samples with prostate intraepithelial neoplasia. High levels of PTOV1 in tumors correlated significantly with proliferative index and localization of PTOV1 in the nucleus. In quiescent cultured prostate tumor cells, PTOV1 localized to the cytoplasm and was excluded from nuclei. After serum stimulation, PTOV1 partially translocated to the nucleus at the beginning of S phase.
Background: PTOV1 was predicted to form alternating alpha helices and beta sheets. Normal prostate expressed low PTOV1 levels. Western blot analysis of prostate tumor tissue detected PTOV1 at an apparent molecular mass of 58 kD. Immunocytochemical analysis detected endogenous PTOV1 in the cytoplasm, concentrated around the nucleus.PTOV1 was overexpressed in 71% of 38 prostate carcinomas and in 80% of samples with prostate intraepithelial neoplasia. High levels of PTOV1 in tumors correlated significantly with proliferative index and localization of PTOV1 in the nucleus. In quiescent cultured prostate tumor cells, PTOV1 localized to the cytoplasm and was excluded from nuclei. After serum stimulation, PTOV1 partially translocated to the nucleus at the beginning of S phase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prostate tumor-overexpressed gene 1 protein homolog
NCBI Official Synonym Full Names
PTOV1, extended AT-hook containing adaptor protein
NCBI Official Symbol
Ptov1
NCBI Protein Information
prostate tumor-overexpressed gene 1 protein homolog
UniProt Protein Name
Prostate tumor-overexpressed gene 1 protein homolog
Protein Family
UniProt Gene Name
Ptov1
UniProt Entry Name
PTVO1_RAT
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Product Notes
The Rat PTOV1 ptov1 (Catalog #AAA9712020) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712020 ELISA Kit recognizes Rat PTOV1. It is sometimes possible for the material contained within the vial of "Prostate Tumor-Overexpressed Gene 1 Protein (PTOV1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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