Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Renal tumor antigen (RAGE) Standard Curve.)
Rat Renal Tumor Antigen (RAGE) ELISA Kit | RAGE elisa kit
Rat Renal Tumor Antigen (RAGE) ELISA Kit
Gene Names
MOK; RAGE; RAGE1; STK30; RAGE-1
Reactivity
Rat
Synonyms
Renal Tumor Antigen (RAGE); Rat Renal Tumor Antigen (RAGE) ELISA Kit; RAGE; MOK; RAGE1; MAPK/MAK/MRK overlapping kinase; renal cell carcinoma antigen (MOK protein kinase); RAGE elisa kit
Reactivity
Rat
Specificity
Rat Renal tumor antigen (RAGE) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RAGE. No significant cross-reactivity or interference between Rat RAGE and analogues was observed.
Sequence Length
389
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Renal tumor antigen (RAGE) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Renal tumor antigen (RAGE) Standard Curve.)
Related Product Information for RAGE elisa kit
Description: This Rat Renal tumor antigen (RAGE) ELISA Kit employs a two-site sandwich ELISA to quantitate RAGE in samples. An antibody specific for RAGE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAGE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAGE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tumor antigens recognized by cytolytic T cells (CTLs) can be classified into 4 groups: shared tumor-specific antigens encoded by MAGE-type genes that are silent in most normal tissues except testis and expressed in many tumors; differentiation antigens that are also expressed in normal cells; antigens resulting from tumor-specific point mutations; and antigens overexpressed in tumor cells. By screening cells expressing both HLA-B7 and kidney tumor cell RNA with autologous CTLs, followed by anchored PCR analysis, Gaugler et al. (1996) isolated a cDNA encoding RAGE. RT-PCR analysis detected expression only in retina and in a number of tumor cell types. One putative RAGE open reading frame encodes a 40-amino acid protein and includes the peptide recognized by HLA-B7-restricted CTLs.
Background: Tumor antigens recognized by cytolytic T cells (CTLs) can be classified into 4 groups: shared tumor-specific antigens encoded by MAGE-type genes that are silent in most normal tissues except testis and expressed in many tumors; differentiation antigens that are also expressed in normal cells; antigens resulting from tumor-specific point mutations; and antigens overexpressed in tumor cells. By screening cells expressing both HLA-B7 and kidney tumor cell RNA with autologous CTLs, followed by anchored PCR analysis, Gaugler et al. (1996) isolated a cDNA encoding RAGE. RT-PCR analysis detected expression only in retina and in a number of tumor cell types. One putative RAGE open reading frame encodes a 40-amino acid protein and includes the peptide recognized by HLA-B7-restricted CTLs.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
MAPK/MAK/MRK overlapping kinase isoform 2
NCBI Official Synonym Full Names
MOK protein kinase
NCBI Official Symbol
MOK
NCBI Official Synonym Symbols
RAGE; RAGE1; STK30; RAGE-1
NCBI Protein Information
MAPK/MAK/MRK overlapping kinase
UniProt Protein Name
MAPK/MAK/MRK overlapping kinase
UniProt Gene Name
MOK
UniProt Synonym Gene Names
RAGE; RAGE1; RAGE-1
UniProt Entry Name
MOK_HUMAN
NCBI Description
This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Dec 2012]
Uniprot Description
MOK: a CMGC protein kinase of the RCK family. Possesses a Thr-Glu-Tyr motif in the activation loop domain, as do classical MAP kinases. Associates with and is apparently stabilized by HSP90.
Protein type: EC 2.7.11.22; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; RCK family
Chromosomal Location of Human Ortholog: 14q32
Cellular Component: cytoplasm
Molecular Function: protein serine/threonine kinase activity; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity
Biological Process: regulation of cell cycle; signal transduction; protein amino acid phosphorylation
Research Articles on RAGE
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Product Notes
The Rat RAGE mok (Catalog #AAA9711988) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711988 ELISA Kit recognizes Rat RAGE. It is sometimes possible for the material contained within the vial of "Renal Tumor Antigen (RAGE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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