Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat RAC-beta serine/threonine-protein kinase (AKT2) Standard Curve.)
Rat RAC-beta Serine/Threonine-Protein Kinase (AKT2) ELISA Kit | AKT2 elisa kit
Rat RAC-beta Serine/Threonine-Protein Kinase (AKT2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat RAC-beta serine/threonine-protein kinase (AKT2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat RAC-beta serine/threonine-protein kinase (AKT2) Standard Curve.)
Background: AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins. Mice lacking Akt2 have a normal body mass, but display a profound diabetic phenotype, indicating that Akt2 plays a key role in signal transduction downstream of the insulin receptor. Mice lacking Akt2 show worse outcome in breast cancer initaited by the large T antigen as well as the neu oncogene.
NCBI and Uniprot Product Information
NCBI Description
may be involved in phosphatidylinositol 3-kinase (PI3-K) mediated signaling [RGD, Feb 2006]
Uniprot Description
Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; Oncoprotein; Kinase, protein; AGC group; AKT family
Cellular Component: cell cortex; cytosol; early endosome; intracellular membrane-bound organelle; nucleoplasm; nucleus; plasma membrane; vesicle
Molecular Function: ATP binding; protein binding; protein kinase activity; protein kinase C binding; protein serine/threonine kinase activity
Biological Process: carbohydrate transport; cellular response to hormone stimulus; cellular response to insulin stimulus; glucose metabolic process; glycogen biosynthetic process; insulin receptor signaling pathway; intracellular protein transport across a membrane; multicellular organismal development; myelin maintenance in the peripheral nervous system; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of RNA splicing; peptidyl-serine phosphorylation; positive regulation of cell migration; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of positive chemotaxis; positive regulation of protein amino acid phosphorylation; positive regulation of signal transduction; positive regulation of transcription from RNA polymerase II promoter; positive regulation of vesicle fusion; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of translation; response to insulin stimulus; response to muscle activity; response to nutrient levels; response to osmotic stress
Research Articles on AKT2
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Product Notes
The Rat AKT2 akt2 (Catalog #AAA9711879) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711879 ELISA Kit recognizes Rat AKT2. It is sometimes possible for the material contained within the vial of "RAC-beta Serine/Threonine-Protein Kinase (AKT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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