Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dopamine transporter (DAT) Standard Curve.)
Rat Dopamine Transporter (DAT) ELISA Kit | DAT elisa kit
Rat Dopamine Transporter (DAT) ELISA Kit
Gene Names
Slc6a3; Dat1
Reactivity
Rat
Synonyms
Dopamine Transporter (DAT); Rat Dopamine Transporter (DAT) ELISA Kit; SLC6A3; DAT; DAT1; DAT elisa kit
Reactivity
Rat
Specificity
Rat Dopamine transporter (DAT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC6A3. No significant cross-reactivity or interference between Rat SLC6A3 and analogues was observed.
Sequence Length
619
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dopamine transporter (DAT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dopamine transporter (DAT) Standard Curve.)
Related Product Information for DAT elisa kit
Description: This Rat Dopamine transporter (DAT) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A3 in samples. An antibody specific for SLC6A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC6A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dopamine transporter is a membrane-spanning protein that binds the neurotransmitter dopamine; DAT provides the primary mechanism through which dopamine is cleared from synapses, transporting dopamine from the synapse into a neuron. DAT is present in the peri-synaptic area of dopaminergic neurons in areas of the brain where dopamine signaling is common. DAT is implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder, bipolar disorder, clinical depression, and alcoholism. DAT is located on human chromosome 5, consists of 15 coding exons, and is roughly 64 kbp long. Evidence for the associations between DAT and dopamine related disorders has come from a genetic polymorphism in the DAT, which influences the amount of protein expressed.
Background: Dopamine transporter is a membrane-spanning protein that binds the neurotransmitter dopamine; DAT provides the primary mechanism through which dopamine is cleared from synapses, transporting dopamine from the synapse into a neuron. DAT is present in the peri-synaptic area of dopaminergic neurons in areas of the brain where dopamine signaling is common. DAT is implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder, bipolar disorder, clinical depression, and alcoholism. DAT is located on human chromosome 5, consists of 15 coding exons, and is roughly 64 kbp long. Evidence for the associations between DAT and dopamine related disorders has come from a genetic polymorphism in the DAT, which influences the amount of protein expressed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sodium-dependent dopamine transporter
NCBI Official Synonym Full Names
solute carrier family 6 member 3
NCBI Official Symbol
Slc6a3
NCBI Official Synonym Symbols
Dat1
NCBI Protein Information
sodium-dependent dopamine transporter
UniProt Protein Name
Sodium-dependent dopamine transporter
Protein Family
UniProt Gene Name
Slc6a3
UniProt Synonym Gene Names
DA transporter; DAT
NCBI Description
acts as a sodium-dependent dopamine transporter; may play a role in regulation of dopamine metabolism and signaling [RGD, Feb 2006]
Uniprot Description
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Research Articles on DAT
Similar Products
Product Notes
The Rat DAT slc6a3 (Catalog #AAA9711865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711865 ELISA Kit recognizes Rat DAT. It is sometimes possible for the material contained within the vial of "Dopamine Transporter (DAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
