Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) Standard Curve.)
Rat Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) ELISA Kit | TAP2 elisa kit
Rat Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) ELISA Kit
Gene Names
Tap2; Cim; Abcb3
Reactivity
Rat
Synonyms
Transporter 2; ATP-Binding Cassette; Sub-Family B (MDR/TAP) (TAP2); Rat Transporter 2; Sub-Family B (MDR/TAP) (TAP2) ELISA Kit; TAP2; DAAP-57C1.2; ABC18; ABCB3; APT2; D6S217E; PSF2; RING11; ABC transporter; MHC 2; ATP-binding cassette; sub-family B (MDR/TAP); member 3; antigen peptide transporter 2; peptide supply factor 2; peptide tr; TAP2 elisa kit
Reactivity
Rat
Specificity
Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAP2. No significant cross-reactivity or interference between Rat TAP2 and analogues was observed.
Sequence Length
703
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) Standard Curve.)
Related Product Information for TAP2 elisa kit
Description: This Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP2 in samples. An antibody specific for TAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The membrane-associated protein encoded by TAP2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]
Background: The membrane-associated protein encoded by TAP2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
antigen peptide transporter 2
NCBI Official Synonym Full Names
transporter 2, ATP binding cassette subfamily B member
NCBI Official Symbol
Tap2
NCBI Official Synonym Symbols
Cim; Abcb3
NCBI Protein Information
antigen peptide transporter 2
UniProt Protein Name
Antigen peptide transporter 2
Protein Family
UniProt Gene Name
Tap2
UniProt Synonym Gene Names
Abcb3; Mtp2; APT2
UniProt Entry Name
TAP2_RAT
NCBI Description
transports peptides into the ER lumen for binding with MHC class I molecules; plays a role in antigen processing and presentation [RGD, Feb 2006]
Uniprot Description
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin ().
Research Articles on TAP2
Similar Products
Product Notes
The Rat TAP2 tap2 (Catalog #AAA9711800) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711800 ELISA Kit recognizes Rat TAP2. It is sometimes possible for the material contained within the vial of "Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
