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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Tenascin (TNC) Standard Curve.)

Rat Tenascin (TNC) ELISA Kit | TNC elisa kit

Rat Tenascin (TNC) ELISA Kit

Gene Names
TNC; GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
Reactivity
Rat
Synonyms
Tenascin (TNC); Rat Tenascin (TNC) ELISA Kit; TNC; HXB; MGC167029; TN; hexabrachion (tenascin C; cytotactin); hexabrachion (tenascin); tenascin C (hexabrachion); TNC elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
Rat Tenascin (TNC) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNC. No significant cross-reactivity or interference between Rat TNC and analogues was observed.
Sequence Length
2201
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Tenascin (TNC) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Tenascin (TNC) Standard Curve.)
Related Product Information for TNC elisa kit
Description: This Rat Tenascin (TNC) ELISA Kit employs a two-site sandwich ELISA to quantitate TNC in samples. An antibody specific for TNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Tenascin (TNC) encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tenascin
NCBI Official Synonym Full Names
tenascin C
NCBI Official Symbol
TNC
NCBI Official Synonym Symbols
GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
NCBI Protein Information
tenascin
UniProt Protein Name
Tenascin
Protein Family
UniProt Gene Name
TNC
UniProt Synonym Gene Names
HXB; TN; TN-C
UniProt Entry Name
TENA_HUMAN

NCBI Description

This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

Uniprot Description

TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular matrix; extracellular space; focal adhesion; membrane; extracellular region; basement membrane

Molecular Function: syndecan binding

Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; response to ethanol; wound healing; response to mechanical stimulus; positive regulation of cell proliferation; response to wounding; negative regulation of cell adhesion; cell adhesion; axon regeneration in the peripheral nervous system; neuromuscular junction development; odontogenesis of dentine-containing teeth

Disease: Deafness, Autosomal Dominant 56

Research Articles on TNC

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Product Notes

The Rat TNC tnc (Catalog #AAA9711680) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711680 ELISA Kit recognizes Rat TNC. It is sometimes possible for the material contained within the vial of "Tenascin (TNC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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