Rabbit CDK5RAP2 Polyclonal Antibody | anti-CDK5RAP2 antibody

CDK5RAP2 Antibody

Cat. Num. AAA9630995

• Gene Names: CDK5RAP2; C48; MCPH3; Cep215; KIAA1633; DKFZp686B1070; DKFZp686D1070
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CDK5RAP2; Polyclonal Antibody; CDK5RAP2 Antibody; C48; CDK5 activator-binding protein C48; CDK5 regulatory subunit associated protein 2; CDK5 regulatory subunit-associated protein 2; centrosomal protein 215 kDa; Centrosome-associated protein 215; CEP215; CK5P2_HUMAN; MCPH3; anti-CDK5RAP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CDK5RAP2 Antibody detects endogenous levels of CDK5RAP2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CDK5RAP2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CDK5RAP2.
• Conjugation: Unconjugated
• Post Translational Modifications: Phosphorylated in vitro by CDK5.
• Subunit Structure: Interacts with CDK5R1 (p35 form) (By similarity). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. May form a complex with CDK5R1 and CDK5 (By similarity). Interacts with pericentrin/PCNT; the interaction is leading to centrosomal and Golgi localization of CDK5RAP2 and PCNT. Interacts with AKAP9; the interaction targets CDK5RAP2 and AKAP9 to Golgi apparatus. Interacts with MAPRE1; the interaction is direct and targets CDK5RAP2 and EB1/MAPRE1 to microtubule plus ends. Interacts with TUBG1; the interaction is leading to the centrosomal localization of CDK5RAP2 and TUBG1. Interacts with TUBGCP3. Interacts with CALM1. Interacts with CDC20. Interacts with CEP68; degradation of CEP68 in early mitosis leads to removal of CDK5RAP2 from the centrosome which promotes centriole disengagement and subsequent centriole separation. Interacts with NCKAP5L. Forms a pericentrosomal complex with AKAP9, MAPRE1 and PDE4DIP isoform 13/MMG8/SMYLE; within this complex, MAPRE1 binding to CDK5RAP2 may be mediated by PDE4DIP. Interacts with LGALS3BP; this interaction may connect the pericentrosomal complex to the gamma-tubulin ring complex (gamma-TuRC) to promote microtubule assembly and acetylation.
• Tissue Specificity: Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
• Function: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes. In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery. Required for the recruitment of AKAP9 to centrosomes. Plays a role in neurogenesis (By similarity).
• Note: Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends in the presence of EB1/MAPRE1. Localization to centrosomes versus Golgi apparatus may be cell type-dependent. For instance, in SK-BR-3 and HEK293F cells, localizes to centrosomes but not to the Golgi apparatus (PubMed:29162697).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CDK5RAP2 antibody

Cytoplasm; Cytoskeleton; Microtubule organizing center; Centrosome; Golgi apparatus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 58535453
• NCBI GeneID: 55755
• NCBI Accession #: NP_001011649.1
• NCBI GenBank Nucleotide #: NM_001011649.1
• UniProt Accession #: Q96SN8; Q05CZ3; Q5CZA0; Q5JTU8; Q5JTU9; Q66GT8; Q6MZT4; Q7Z3M0
• Molecular Weight: 35,755 Da
• NCBI Official Full Name: CDK5 regulatory subunit-associated protein 2 isoform b
• NCBI Official Synonym Full Names: CDK5 regulatory subunit associated protein 2
• NCBI Official Symbol: CDK5RAP2
• NCBI Official Synonym Symbols: C48; MCPH3; Cep215; KIAA1633; DKFZp686B1070; DKFZp686D1070
• NCBI Protein Information: CDK5 regulatory subunit-associated protein 2; OTTHUMP00000022800; OTTHUMP00000215329; centrosomal protein 215 kDa; centrosome-associated protein 215; CDK5 activator-binding protein C48
• UniProt Protein Name: CDK5 regulatory subunit-associated protein 2
• Protein Family: CDK5 regulatory subunit-associated protein
• UniProt Gene Name: CDK5RAP2
• UniProt Synonym Gene Names: CEP215; KIAA1633
• UniProt Entry Name: CK5P2_HUMAN

Uniprot Description

CDK5RAP2: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: Golgi apparatus; spindle pole; centrosome; microtubule; pericentriolar material; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; cell junction; cytosol

Molecular Function: calmodulin binding; tubulin binding; protein binding; microtubule binding; protein kinase binding

Biological Process: regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of centriole replication; chromosome segregation; negative regulation of neuron differentiation; establishment of mitotic spindle orientation; neurogenesis; centrosome organization and biogenesis; mitotic cell cycle; brain development; G2/M transition of mitotic cell cycle; microtubule bundle formation

Disease: Microcephaly 3, Primary, Autosomal Recessive