Rabbit anti-Human, Mouse CARD14 Polyclonal Antibody | anti-CARD14 antibody

CARD14 Antibody

Cat. Num. AAA9630960

• Gene Names: CARD14; PRP; PSS1; BIMP2; CARMA2; PSORS2
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CARD14; Polyclonal Antibody; CARD14 Antibody; Bcl10 interacting MAGUK protein 2; Bimp 2; Bimp2; CAR14_HUMAN; CARD 14; CARD containing MAGUK 2 protein; CARD containing MAGUK protein 2; Card maguk protein 2; CARD-containing MAGUK protein 2; Carma 2; Carma2; Caspase recruitment domain containing protein 14; Caspase recruitment domain family member 14; Caspase recruitment domain protein 14; Caspase recruitment domain-containing protein 14; PRP; PSORS2; PSS1; anti-CARD14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CARD14 Antibody detects endogenous levels of CARD14.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CARD14 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CARD14.
• Conjugation: Unconjugated
• Similarity: A linker region between the coiled-coil and PDZ region holds the protein in an inactive state (PubMed:27071417).
• Subunit Structure: Interacts (via CARD domain) with BCL10 (via CARD domain). Forms a complex with MALT1 and BCL10; resulting in the formation of a CBM (CARD14-BLC10-MALT1) complex. Interacts with TRAF2, TRAF3 and TRAF6.
• Tissue Specificity: Isoform 1 is detected in placenta and epidermal keratinocytes. Isoform 2 is detected in leukocytes and fetal brain.
• Function: Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14-induced activation of NF-kappa-B and p38/JNK MAP kinases. May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.Not able to activate the inflammatory transcription factor NF-kappa-B and may function as a dominant negative regulator.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CARD14 antibody

Cytoplasm; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 384229040
• NCBI GeneID: 79092
• NCBI Accession #: NP_001244899.1
• NCBI GenBank Nucleotide #: NM_001257970.1
• UniProt Accession #: Q9BXL6; Q9BVB5
• Molecular Weight: 113,270 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 14 isoform 3
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 14
• NCBI Official Symbol: CARD14
• NCBI Official Synonym Symbols: PRP; PSS1; BIMP2; CARMA2; PSORS2
• NCBI Protein Information: caspase recruitment domain-containing protein 14; carma 2; card-maguk protein 2; CARD-containing MAGUK protein 2; bcl10-interacting maguk protein 2
• UniProt Protein Name: Caspase recruitment domain-containing protein 14
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD14
• UniProt Synonym Gene Names: CARMA2; Carma 2
• UniProt Entry Name: CAR14_HUMAN

NCBI Description

This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

CARD14: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; plasma membrane

Molecular Function: CARD domain binding

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis; activation of NF-kappaB transcription factor

Disease: Psoriasis 2; Pityriasis Rubra Pilaris