Rabbit anti-Human ANK3 Polyclonal Antibody | anti-ANK3 antibody

ANK3 Antibody

Cat. Num. AAA9630763

• Gene Names: ANK3; MRT37; ANKYRIN-G
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ANK3; Polyclonal Antibody; ANK3 Antibody; ANK-3; ANK3_HUMAN; ankyrin 3 (G); Ankyrin 3; node of Ranvier (ankyrin G); node of Ranvier; Ankyrin G; Ankyrin G119; Ankyrin-3; Ankyrin-G; brain specific ankyrin G; CHANK3; FLJ45464; OTTHUMP00000217458; OTTHUMP00000217575; RP11 369L1.1; anti-ANK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ANK3 Antibody detects endogenous levels of ANK3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ANK3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ANK3.
• Conjugation: Unconjugated
• Similarity: The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin (By similarity).
• Subunit Structure: Directly interacts with DMD and betaDAG1. This interaction does not interfere with binding between DMD and betaDAG1. It is also required for DMD and betaDAG1 retention at costameres (By similarity). Interacts (via N-terminal ANK repeats) with SCHIP1 isoform 5 (via C-terminus); this interaction is required for the localization at axon initial segments (AISs) and nodes of Ranvier (NRs) (By similarity). May be a constituent of a NFASC/NRCAM/ankyrin G complex. Interacts with RHBG. Interacts with PLEC and FLNC. Interacts with KCNA1; this inhibits channel activity. Interacts (via ANK repeats) with IQCJ-SCHIP1; required for IQCJ-SCHIP1 localization at axon initial segments (AIS) and nodes of Ranvier. Interacts with SCHIP1. Interacts with SCN5A.
• Tissue Specificity: Expressed in brain, neurons, muscles and other tissues.
• Function: In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption.May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.
• Note: In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes.Cytoplasm>Cytoskeleton. Golgi apparatus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ANK3 antibody

Cytoplasm; Cytoskeleton; Cell projection; Axon; Cell membrane; Sarcolemma; Cell junction; Synapse; Postsynaptic cell membrane; Lysosome; Cell membrane; Sarcolemma; T-tubule; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 32967599
• NCBI GeneID: 288
• NCBI Accession #: NP_001140.2
• NCBI GenBank Nucleotide #: NM_001149.3
• UniProt Accession #: Q12955; Q13484; Q5CZH9; Q5VXD5; Q7Z3G4; Q9H0P5; B1AQT2; B4DIL1; E9PE32
• Molecular Weight: 160,947 Da
• NCBI Official Full Name: ankyrin-3 isoform 2
• NCBI Official Synonym Full Names: ankyrin 3, node of Ranvier (ankyrin G)
• NCBI Official Symbol: ANK3
• NCBI Official Synonym Symbols: MRT37; ANKYRIN-G
• NCBI Protein Information: ankyrin-3; ankyrin G119
• UniProt Protein Name: Ankyrin-3
• Protein Family: Ankyrin
• UniProt Gene Name: ANK3
• UniProt Synonym Gene Names: ANK-3
• UniProt Entry Name: ANK3_HUMAN

NCBI Description

Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

ANK3: In skeletal muscle, required for costamere localization of DMD and betaDAG1. Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Genetic variations in ANK3 are associated with autism spectrum disorders susceptibility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: Golgi apparatus; tight junction; costamere; cell surface; sarcoplasmic reticulum; endoplasmic reticulum; basolateral plasma membrane; lysosome; paranode region of axon; T-tubule; dendrite; Z disc; postsynaptic membrane; plasma membrane; basal plasma membrane; neuromuscular junction; sarcolemma; lateral plasma membrane

Molecular Function: protein binding, bridging; protein binding; spectrin binding; structural constituent of cytoskeleton; cadherin binding; cytoskeletal protein binding

Biological Process: axon guidance; regulation of potassium ion transport; cytoskeletal anchoring; generation of action potential; signal transduction; positive regulation of homotypic cell-cell adhesion; establishment of protein localization; cytokinesis after mitosis; plasma membrane organization and biogenesis; axonogenesis; Golgi to plasma membrane protein transport; positive regulation of membrane potential; neuromuscular junction development

Disease: Mental Retardation, Autosomal Recessive 37